Fluorescent Probes For Biological Molecules,Ions And Its Application In Living Cell Imaging

Fluorescent probes have been research in biologically relevant molecules such as amino acids,peptides,oxides and superoxide,ions such as metal ions,anions,and H⁺of cellular and cytoplasmic in recent years,to make it more and more widely applied in the field of biology and medicine.Application of fluorescence microscopic imaging technology to identify the distribution and dynamic changes of biological related molecules and ions in the cells,realizing real-time online,sensitive and rapid detection by cell imaging,providing visual information for the study of the biological related molecules and ions influence on the functions of the human organism.The main contents of this thesis are as follows:1.Using spectrum analysis study the probe 1⁵ of the research group synthesis for recognition property of biological related molecules and ions.The possible stoichiometric ratio,the locus of action and the mechanism were investigated by means of ¹ HNMR,MS,IR and ITC.The coumarin aldehyde-amino thiourea probe 1 has a good selectivity to Zn²⁺and AcO^-in DMSO/H₂O medium,The addition of Zn²⁺made probe from green fluorescence to strong blue fluorescence;The coumarin triazole probe 2 has a fluorescence quenching response to Fe³⁺and Cu²⁺in C₂H₅OH/H₂O medium,the recognition is based on the mechanism of photoinduced electron transfer?PET?,the probe 2 responses to Fe³⁺by dynamic quenching;The quinolines probe 3 detects pH,H₂O₂ and GSH by different role models in CH₃CN/H₂O medium,respectively;The rhodamine-NBD?7-nitro-1,2,3-benzoxadiazole?probe 4 with ratio fluorescence recognizes Hg²⁺,fluorescence enhancement recognizes Cu²⁺and fluorescence quenching recognizes F^-,the probe detects Hg²⁺and Cu²⁺by the mechanism of fluorescence resonance energy transfer?FRET?and intramolecular charge transfer?ICT?,respectively,and detect F^-by two kinds of mechanism combination of ICT and PET;The rhodamine-naphthalene isocyanate probe 5 has a good recognition to Fe³⁺and Cr³⁺in CH₃CN/H₂O medium is based on FRET and rhodamine ring opening mechanism,and detects I^-by PET mechanism.2.The fluorescent imaging of PC3 cells of coumarin,quinoline and rhodamine fluorescent probes 1 to 6 was studied,for detecting the specific molecular and ion by fluorescence microscopic imaging technology.Cells present green fluorescence after probe 1 incubation,then after Zn²⁺incubation,appearing blue fluorescence imaging under the blue channel;The probe 2can detect Fe³⁺by intracellular blue fluorescence quenching imaging;The probe 3 can qualitatively detect pH,H₂O₂ and GSH in PC3 cells,respectively,multi-target detection is realized;The probe 4 can detect Hg²⁺under both of green and red channels and Cu²⁺under green channel,multi-channel detection is realized;The probe5 and rhodamine-naphthalene isothiocyanates probe 6 detect Fe³⁺and Al³⁺under both of green and red channels,respectively.