Extraction Of Polyphenol From Strawberry And Its Application In Whitening Cosmetics

Strawberry is a perennial herb.In recent years,strawberry production in China has developed rapidly,whether it is planting area or output ranks first in the world.Domestic strawberry storage and preservation of many studies,but the strawberry physiological activity rarely mentioned,research and development of strawberry polyphenol products with broad market value.Add natural active ingredients of cosmetics with less irritation,good permeability and significant efficacy,etc.,loved by more and more people.Strawberry polyphenols have a strong antioxidant scavenging free radicals,anti-tumor,anti-bacterial anti-inflammatory,anti-aging,prevention and treatment of cardiovascular disease,nourishing the gastrointestinal tract and nutrition whitening skin and so on.Strawberry polyphenols applied to whitening cosmetics is a scientific basis for the attempt with broad market prospects.In this paper,ultrasonic extraction of strawberry polyphenols by ultrasonic assisted extraction.The optimum process parameters were selected according to the single factor experiment and the response surface optimization experiment.Ultrasonic extraction extraction method: ethanol concentration 67%,ultrasonic time 35 min,ratio of material to liquid 1:40.The adsorption efficiency and the analytical rate of the six macroporous resins were compared.The adsorption of NKA-?macroporous resin was better and the purification of the crude extract of strawberry polyphenol was better.The adsorption parameters of NKA-?macroporous resin were determined by the static adsorption analysis of NKA-?macroporous resin.The pH value of the adsorbate was 3,the pH value of the solution was 3,the concentration of ethanol was 50%.Determination of NKA-?Macroporous Resin by HPLC.Sephadex LH-20 further purified the components of strawberry polyphenols,HPLC detection of different concentrations of ethanol eluent obtained polyphenolic material species.Strawberry extract Strawberry polyphenols for in vitro efficacy and determination of human safety.The effects of three different drying methods on the activity of polyphenols in strawberry were studied in fresh strawberry,freeze-drying and drying at 45 ?.The contents of total phenolics in the three samples were not significantly different,and the contents of procyanidins and total flavonoids in fresh and dried samples were inversely proportional.The freeze-dried strawberry powder had a long shelf life and good antioxidant effect.The strongest tyrosinase inhibition rate The results showed that there was no significant difference in the tyrosinase inhibition rate.Through the Chinese women's subjects 8 weeks of eye human trial test,rich in strawberry polyphenols of the human skin is safe;the test substance has to reduce the spot and non-spot parts melanin content,improve the stain site Skin brightness and improved skin color of the stain site.In summary,the strawberry polyphenol active material was prepared successfully.The results showed that strawberry extract strawberry polyphenols had good antioxidant ability and whitening effect,which provided scientific basis for its application in cosmetics.