Fabrication And Properties Of (PLGA/HA)/(Collagen/Amoxicillin) Bone Guided Regeneration Nanofibers Membrane By Coaxial Electrospinning

As the demand of oral clinical is increasing,guide bone regeneration membrane was required not only the blocking effect but also a variety of functions,such as guided bone growth,drug released slowly.The multilayer guided bone regeneration membrane which was prepared by to meet the above functions was produced by electrospinning,however,the interlayer binding force is too poor to fall off and the main degradation of PCL(polycaprolactone)is slow,that does not match with the oral repair period of treatment.Guided bone regeneration nanofibers membrane was produced by coaxial electrospinning in this paper.In this paper,core-shell structure(PLGA/HA)/(Collagen/Amoxicillin)guided bone regeneration nanofibers membrane was produced by coaxial electrospinning,through the release of anti-inflammatory analgesic which is in the sheath of Collagen/Amoxicillin(Collagen/Amoxicillin),that can promote wound healing;after the shell degradation,PLGA/HA(polylactic acid-glycolic acid/hydroxyapatite)as the core layer also can block fibroblasts long bone defect,which HA can promote bone growth.The preparation process of core-shell structure nanofibers membrane was optimized and the composition,morphology and core-shell structure of nanofibers membrane were characterized,and the degradation performance in vitro,hydrophilic properties and the drug release properties in vitro of nanofibers membrane were researched.The following results were obtained:The results of degradation in vitro show that the weightlessness rate of first two weeks is increased rapidly in the period of nanofibers membrane degradation,then slow degradation.After 8w,the nanofibers began to break.After composite nanofibers membrane degradation,its surface appear apatite crystals,namely mineralization occurs,and this phenomenon is beneficial to bone growth.Due to the introduction of the Collagen,the wettability of composite nanofibers membrane became from hydrophobic to hydrophilic,which the water contact angle decreased from 119 ° to 55.5 °,the porosity rate was above 90%,the size of pore was micro-nano.As the degradation of nanofibers membrane also gradually became hydrophobic,that can ensure block role of fibroblasts.The results of the SBF water contact angle showed that,SBF wetting composite nanofiber membrane is very good to conducive to cell adhesion and growth.In vitro drug release results show that,as time extended and the degradation of nanofibers membrane,nanofibers membrane of different collagen concentration appeared drug release behavior which was initial burst release and then release slowly.The time of drug release completely was within 48 h to prevent the drug resistance.Therefore,the guided bone regeneration membrane can satisfied by the current clinical requirements in this paper.