| Chemiluminescence（CL）has been one of the most important analytic methods by virtue of the unique advantages of CL,such as low interference of scattered light background,high sensitivity,wide linear range,fast analysis speed and simple miniaturized apparatus.Luminol is a kind of the most frequently and widely used CL reagents because of its good water solubility and relatively high luminescence quantum yield.For the low speed of the oxidant reaction of luminol,the luminol CL systems usually need catalytic to accelerate the reaction speed.The commonly used catalytic are gold nanoparticles,metal oxide nanoparticles and so on.Metal-organic frameworks（MOFs）are the member of organic-inorganic hybrid materials,which are comprised by metal ion and organic ligand via moderate coordination bands.MOFs have good comprehensive properties of organic and inorganic materials,and MOFs display excellent adsorption and separation,optical and catalytic function characteristics.Moreover,benefit from the distinct properties of MOFs,such as orderly tunable pores,ultrahigh specific surface area and unsaturated metal sites,MOFs had been widely utilized in hydrogen storage,catalysis,analysis,and so on.However,the application of MOFs as catalyst in CL is rarely reported.In this paper,we selected two kinds of MOFs with catalytic activity to constructed new CL methods for sensitively detection of cysteine,hydrogen peroxide and glucose.The detailed results are summarized as follows:（1）Co-MOFs-luminol CL system was used to detection of cysteine.We selected Co-metal-organic frameworks（Co-MOFs）,which transformed from Zn-metallogels（Zn-MOGs）,catalyze luminol CL without extra oxidants.The study found that Co-MOFs possessed stronger catalytic activity than Co-MOFs（H）synthesized form hydrothermal method or dissolved cobalt ion.By adding cysteine,the CL emission of luminol-Co-MOFs system was further enhanced.The CL intensity was proportional to the concentration of cysteine in the range of 2-10 μM,and the detection limit was 0.49 μM（3S/N）.Based on this phenomenon,a simple and sensitive CL assay was developed for quantitative determination of cysteine.Moreover,the established method was applied to the determination of cysteine in commercially available pharmaceutical injections.（2）Fe3O4/MIL-101（Fe）-luminol CL system was used to detection of hydrogen peroxide and glucose.Without the addition of oxidant agent,Fe3O4/MIL-101（Fe）could react with dissolved oxygen to yield active oxygen radicals,which taking advantage of the synergistic catalytic activity of Fe3O4/MIL-101（Fe）,then luminol was oxidized by active oxygen radicals to generate CL.For H2O2 could increase the CL intensity of Fe3O4/MIL-101（Fe）-luminol system and the glucose oxidation reaction by glucose oxidase could produce H2O2,the Fe3O4/MIL-101（Fe）-luminol system was used for quantitative determination of H2O2 and glucose.Under optimal conditions,the linear ranges of H2O2 and glucose were 5 nM-150 nM and 5 nM-100 nM respectively,and the LOD of H2O2 and glucose were 3.7 nM and 4.9 nM.Furthermore,the present approach was applied to quantitative determination of H2O2 in medical disinfectant and glucose in human serum samples with satisfactory results. |
