| Modern medicine is in the direction of regeneration and reconstruction of damaged human tissue or organs,restore the body to enhance physical function,minimally invasive treatment and other directions to flourish.Traditional non-life medical metal,polymer,bioceramics and other materials have been unable to meet the medical development requirements.Therefore,it is of great significance to strengthen the clinical medical research and application promotion of biomedical materials.New biodegradable hydrogel as a biodegradable polymer material can be used as a drug carrier,the drug released into the human body to achieve therapeutic effect.Can also be on the cell,tissue or organ diagnosis and treatment,replacement repair and induced regeneration,biological materials must be directly or indirectly with the human body cells,blood or tissue in contact with each other.The purpose of this evaluation is to study CMCS-gel & CMCS-PLA-gel’s blood compatibility,cell compatibility,histocompatibility and in vivo drug release studies.In this study,we studied the effects of CMCS-gel& CMCS-PLA-gel on mouse fibroblasts(L-929)(1)MTT assay was used to detect the effect of material on the survival and growth of mouse fibroblasts(L-929).(2)Agar cover method was performed by observing cell shedding or cell lysis(3)LDH method was used to determine the cell membrane integrity and mitochondrial metabolism and functional status of the reaction material leaching solution on mouse fibroblasts(L-929)cytotoxicity effects on mouse fibroblasts(L-929).The experimental results show that the leaching solution of these two novel biodegradable hydrogels has no toxic effect on cell proliferation and has good cytocompatibility.The hemolysis rate of these two new biodegradable hydrogels was less than 5%,and the coagulation time experiment,plasma calcium recovery time experiment and dynamic clotting time showed no coagulation,anticoagulant performance is good,with good blood compatibility.By implanting the material into SD rats subcutaneously,the degradation in vivo was observed by the naked eye and the pathological sections of HE were observed under electron microscope.The inflammatory reaction was confirmed by the HE staining.It was concluded that the lumps became smaller and localized at the 5th day,indicating that the material in the progressive degradation of absorption,but there are a large number of inflammatory cell infiltration,mainly macrophages,a small amount of plasma cells and lymphocytes;10d mass is still but the inside of the hydrogel is no longer,the inflammatory response;The 19 th day rats returned to normal subcutaneous.The results showed that the growth rate of paclitaxel-loaded carboxymethyl chitosan hydrogel did not increase in the initial release of paclitaxel,and the growth rate increased slowly after 7 days of release.After 30 days of release,the cumulative release rate can reach about 50%.The paclitaxel-loaded polylactic acid-carboxymethyl chitosan hydrogel has no synaptic process at the initial stage of release,and the cumulative concentration increases slowly,and the growth rate increases after 7 days The After 30 days of release,the cumulative release rate can reach about 45%.The results showed that the two hydrogels containing hydrophobic drug paclitaxel can achieve slow and sustained release,which has a good effect on reducing the adverse effects of drugs.The results of this study showed thatCMCS-gel&CMCS-PLA-gel have good blood compatibility,compatibility,organizational compatibility,and provide a preliminary reference for the feasibility of the drug release carrier,and its further modification of its biocompatibility of the need for more in-depth study. |
