Surface Patterning Of SEBS And Research On Its Hemocompatibility

2-Gluconamidoethyl methacrylate(GAMA)containing double bond was synthesized by using D-(+)-glucono-1,5-lactone and 2-aminoethyl methacrylate hydrochloride as raw materials.The chemical structures of the GAMA were characterized by Fourier transform infrared spectroscopy(FTIR)and proton nuclear magnetic resonance spectroscopy(1H NMR).GAMA modified styrene-ethylene-butylene-styrene(SEBS)was fabricated via a technique of ultraviolet(UV)induced surface graft polymerization.Attenuated total reflectance Fourier transform infrared spectroscopy(ATR-FTIR)and X-ray photoelectron spectroscopy(XPS)confirmed the functionalized SEBS membrane structures.The hydrophilicity and hemocompatibility of the modified SEBS surface were evaluated by means of static contact angle,protein adsorption,platelet adhesion and hemolytic rate.The even coverage of the surface by well-defined PGAMA brushes enabled SEBS films to exhibit excellent resistance to protein adsorption and platelet adhesion,better hydrophilicity as well as low hemolysis ratio.The hydrophilic layer PAMPS molecular brush was grafted on the SEBS by UV-induced surface graft polymerization to inhibit protein adsorption and blood cells adhesion.Then,we built a binary polymer-patterned hydrophilic surface by fabricating PGAMA-patterned domains on the PAMPS layer with photomask-assistant photolithography,followed by generation of phytohemagglutinin(PHA)arrays through carbohydrate-lectin reaction,besides,the PHA arrays had a tunable size as well as uniform and closely structure.Considering the specific recognition interactions between PHA and red blood cells(RBCs),the blood cells adhesion experiments suggested that the PHA arrays induced nearly no platelets adhere on the patterned surface,but showed high capability to capture RBCs,in addition,the captured RBCs maintain cellular integrity and function.Therefore a newly platform to capture RBCs from the blood harmlessly was provided.The first layer of polyethylene glycol(PEG)molecular brush was grafted on the SEBS surface by UV-induced surface graft polymerization.Then,on the basis of the first layer molecular brush,a binary three-dimensional patterned hydrogel with clear and uniform structure was constructed by combining UV-induced surface graft polymerization and photomask-assistant photolithography.ATR-FTIR and XPS measurements confirmed the functionalized SEBS membrane structures.The surface morphology of the system was observed by polarizing microscope(POM)and scanning electron microscopy(SEM).The hydrophilicity and hemocompatibility of the modified SEBS surface were evaluated by means of static contact angle,protein adsorption,platelet adhesion and red blood cells adhesion.It was found that the first hydrophilic layer of PEG molecular brush could effectively resist the adhesion of protein and blood cells.Due to the hydrophilic surface and the unique topological structure of the hydrogel,it also inhibited the protein adsorption and blood cells adhesion.Therefore,the patterned system exhibited excellent resistance to protein non-specific adsorption and blood cells adhesion,the blood compatibility of SEBS was improved.