The Preparation Technology And Biological Efficiency Of Modified HLC-Fe

At present,iron deficiency anemia(IDA)was one of the most serious diseases which affects human health around the world.It was due to the storage of iron in the body that was not able to meet the needs of normal erythropoiesis.According to the world health organization report,more than 20%of the world's people have varying degrees of anemia.Different generations of iron supplements have been developed,including FeSO4,ferrous succinate and iron amino acid chelate et al.They are all known to be rich in iron,but the bioavailability of iron was poor because of their side effects to the body.To develop an efficiency and safety iron supplement,The HLC with good biocompatibility was used as raw material and FeSO4 used as ligand in this experiment,thiolated of human-like collagen iron complex(SH-HLC-Fe)was prepared by technology improvement.The synthesis condition of SH-HLC-Fe was optimized in this study.The results demonstrated that the optimization synthesis condition of SH-HLC-Fe were:pH 6.5,temperature 25 ?,m(SH-HLC)/m(Fe2+)4:1,c(SH-HLC)4%(g/mL).The characteristics of HLC,SH-HLC and SH-HLC-Fe were investigated by atomic absorption spectrophotometry(AAS),fourier transform infrared spectroscopy(FTIR)and thermal gravity analysis(TGA).The atomic absorption spectrophotometry data showed that one mole SH-HLC contained about 35.34 mole Fe after the technology improvement.Fourier infrared spectrum results revealed that the iron ion binding sites were mainly concentrated on the protein of N-H group.Also the thermodynamic analysis proved that SH-HLC-Fe complex had good thermal stability.The MTT assay showed that SH-HLC-Fe was non-cytotoxicity and could promote the growth of cells.Quantitative analysis of SH-HLC-Fe digesta which simulated in vitro gastrointestinal digestion were analyzed by the SDS-PAGE coupled with gel filtration chromatography methods.The iron bioavailability of SH-HLC-Fe was evaluated by Caco-2 cells monolayer coupled with in vitro gastrointestinal digestion.Results indicated that the iron bioavailability of SH-HLC-Fe was higher than other iron supplements.Additionally,the iron bioavailability was confirmed to be significantly enhanced by vitamin C.At last,the iron biological efficiency of SH-HLC-Fe was evaluated by iron-deficiency anemia mice model.Results demonstrated that SH-HLC-Fe digesta had higher bioavailability than other iron supplements such as FeSO4,ferrous succinate,ferrous glycine chelate and SH-HLC-Fe.These results indicated that SH-HLC-Fe would be a promising iron supplement in the future.