Preparation Of Biomacromolecularly Imprinted Polymers By Plasma Polymerization

Biomacromolecularly imprinted polymers(BMIPs)are a kind of biomimetic polymer materials,which have a specific recognition ability to the template biological molecules and it is believed to have a broad application prospect in the fields of biological sensors.Compared with the traditional biological ligands,such as antibodies,enzymes,imprinted polymers have some advantages such as the low cost,good stability,autonomous design,long service life and high tolerance to the harsh environment.Therefore,building a biosensor by the use of biomacromolecularly imprinted polymers has already attracted a wide range of attention,which also becomes a new research hotspot.However,because of the unique structures of biological macromolecules,the research progress of its development is still a long way to go.In this paper,we chose protein as the template molecules and chose glycidol and di(ethylene glycol)monovinyl ether as the functional monomers,respectively.With the surface imprinting methods,namely plasma polymerization technique,we deposit thin films on the silicon substrates and the gold substrates,respectively.So we can prepare surface imprinted polymers which may have the specific recognition ability to the bovine serum albumin(BSA)and lysozyme.In the research process of the BMIPs,we characterized the BMIPs’ structures and properties during the different stages of the experimental processes by various analytical methods,including contact angle gonoimeter,Fourier transform infrared spectroscopy(FT-IR)and surface plasmon resonance(SPR).The experimental results showed that the first imprinted polymer thin films prepared by glycidol had a relatively ideal effect.On one hand,the thin films could adsorb sufficient quantities of protein,on the other hand,in the template molecule elution process,the elution ratio could reach about 74%.The second thin films for anti-protein adsorption,which prepared under the condition of pulsed mode,Ton/Toff=1/19 with the monomer of di(ethylene glycol)monovinyl ether,could significantly reduce the adsorption of BSA while had a little adsorption of lysozyme.Through the kinetics analysis of the surface plasmon resonance,we konwed that the 1M NaCl solution had a relatively ideal elution efficiency and was the best way to carry out the elution process of the template molecule currently.From the present results,the BMIPs we prepared now had the specific recognition ability to the template.But if we want to improve the recognition efficiency,We should optimize and improve the deposition conditions of thin films and the elution methods for the template protein.