Preparation Of Plasma Polymerized PEO-like Film And Eveluation Of Anti-Bioadhesion

A great challenge in the applications of artificial biomedical devices such as retrievable vena cava filters (RVCFs), biosensors and surface-based diagnostic devices is the prevention of the nonspecific attachment of proteins, platelets or cells on surfaces. Plasma deposited PEO-like films have been investigated as surface modifications of biomedical materials and these studies show that plasma deposited PEO-like films have good non-fouling properties with significantly resisting the nonspecific attachment of proteins, platelets and cells.In this thesis, PEO-like films on316L SS were prepared by the continuous and pulsed plasma polymerization under different discharge power and the ratio of ethylene glycol dimethyl ether (TEGDMA) to O2. The thickness, composition, structure, film stability, morphology and hydrophility were characterized by step profiler, Fourier transform infrared (FTIR) spectroscopy, X-ray photoelectron spectroscopy (XPS), atomic force microscopy (AFM) and contact angle measurement(CA). At the same time, the proliferation and adhesion of endothelial cell, platelet adhesion and activation in vitro, and fibrinogen (Fg) adsorption were evaluated.The result of AFM showed with the increase of RF discharge power, the ionization increased, and this lead to increase of deposition rate, TEGDMA monomer could be retained less EO functional groups in the PEO-like films which was proved by FTIR and XPS. The results step profiler showed that under high discharge power, the film is relatively smooth. Contact angle test proved that all PEO-like film had better hydrophilicity than SS. Based on the observance of SEM, the swelling phenomenon could be found for the PEO-like films after the films were immersed into water for different days, but the PEO-like films did not appear rupture, spalling and dissolution, which suggested that the films had good stability. The biological evaluation indicated that the PEO-like films could prohibit the platelet adhesion and activation compared to316L stainless steel (SS), but there were not significant differences among the PEO-like films. In vitro endothelial cell adhesion test proved that all PEO-like films could inhibit endothelial cell adhesion, especially the result of1day of cell adhesion demonstrated that the number of the attached cell was the lowest under5power.For PD-PEO series films which were prepared through change the ratio of TEGDMA to O2FTIR and XPS results showed that the content of EO (COC) of the PD-PEO films increased with the increase of the oxygen proportion, which might be due to the new generation of the EO (COC). Under of high content of oxygen, the hydrophilicity of films was the best. All PD-PEO films under the three oxygen ratio had good stability. PD-PEO-1, PD-PEO-2, PD-PEO-3films had good non-fouling properties of adhesion and activation of platelet, fibrinogen (Fg) adsorption, endothelial cells adhesion and proliferation. In addition, PD-PEO-3had the best non-fouling ability which had the highest content of EO. The results demonstrated that anti-bioadhesion of PEO-like film can be enhanced with the increasing of the proportion of oxygen.