Colorimetric Sensor Research Based On The Non-enzyme Self-assembly Nanomaterials And Typical Color Reaction

In recent years,the detection of mercury,sulfur,ascorbic acid and cysteine in the chemical,biological,pharmaceutical and food analysis and other fields is more and more attention.So establish a rapid analysis determination technique for mercury,sulfur,ascorbic acid and cysteine has a great significance.Colorimetric sensing has attracted much attention in the field of analytical chemistry due to the potential for a direct visual readout.It has the advantages of simplicity,rapidity,and cheapness aswell as the fact that there is no requirement for any sophisticated instrumentation.As is well known,the color change of a detection event is a essential for colorimetric sensing.In recent years,due to organic indicator has the advantages such as stability and cheapness,it has emerged in endlessly,and caused the attention of the researchers.This paper was prepared based on self-assembly nanostructures of different metal ions and tetramethylbenzidine system with “On-Off” switching optical function and the interaction between and specific chromogenic reaction,the colorimetric sensors of cations and anions are successfully used in biological molecules and ions.This paper mainly includes the following three aspects:1 The self-assembly nanometer materials was prepared by an organic indicator3’3-5’5-tetramethyl benzidine(TMB),Fe3+and H2O2 were successfully prepared.Based on this,a visual sensing platform for rapid and sensitive detection of cysteine was proposed and explored.The limit of detection was 0.37 μM.In addition,we further validated the sensing mechanism process of the system on cysteine via electrochemical methods and the nanometer materials were characterized by SEM Finally,we successfully designed a two-stage logic gate and actual application in the test paper test.2 The self-assembly nanometer materials was prepared by an organic indicator3’3-5’5-tetramethyl benzidine(TMB)and Ag+ were successfully prepared.Based on this,a visual sensing platform for rapid and sensitive detection of ascorbic acid was proposed and explored.The limit of detection was 0.33 μM.In addition,we further validated the sensing nanometer materials by SEM.Finally,we successfully designed a inhibit logic gate,and build a dipstick test platform.3 An classical Ellman reaction taking advantage of the interaction between organicindicator disulfide nitro benzoic acid(DTNB)and cysteine was successfully prepared.Based on this,we successfully constructed a visual sensing platform for rapid and sensitive detection of Hg2+ and anionic S2-.The detection limit of 0.134 m M and0.442 m M respectively.In addition,we further validated the sensing mechanism process of the Ellman system on Hg2+and anionic S2-via electrochemical methods.In the end we design as two stage logic gate and practical application to the dipstick test.