Enhanced Degradation Of Perilla Stem And Extraction Process Research Of Its' Natural Active Ingredients

Enzymatic hydrolysis is widely concerned because of its specificity and mild conditions;Heteropoly acids are widely used in industrial applications as clean solid acids In this study,the enzymatic hydrolysis technology was applied to the extraction of active components such as ferulic acid,and the secondary residue was obtained by catalytic degradation of heteropoly acid,and the reduction and utilization of the basil,provides a new method for the disposal of abandoned basil and its resource utilization.In order to realize the degradation of plant tissue of Perilla frutescens,this study selected different enzymes to select the high activity complex enzyme preparation(cellulase,Xylanase and laccase).The optimal ratio of compound enzyme was obtained by SPSS and VF software.To improve the dissolution efficiency of ferulic acid in the basil stems,the resource utilization of the purple basil was optimized by using the response surface method.And the kinetic model of reducing s?gar and ferulic acid leaching was established by using the particle diffusion equation.In order to realize the reduction of secondary residue and the efficient degradation of lignin,the solid strong acid phosphotungstic acid was selected as the catalyst.By comparing the catalytic degradation of the secondary residue of basil stupa with different supported catalysts,HPA/TS-1 is a suitable catalyst for the degradation of lignin.The optimum process parameters of lignin degradation by heterogeneous acid were optimized by response surface methodology.The BET(specific surface area)and SEM(electron microscopy)structure and composition of the dexamethasone were studied,and the catalytic performance of the complex enzyme and heteropoly acid was revealed.The conclusions are as follows:(1)By comparing the energy efficiency of leaching of ferulic acid in basil stalksby different enzymes,it was found that the complex enzyme(cellulase,xylanase and laccase)had high catalytic activity,and the complex enzyme was used to hydrolyze the basil,not only can get ferulic acid,also can obtain two high-value active ingredients : caffeic acid and rosmarinic acid.The optimal ratio of compound enzyme was cellulase: laccase: xylanase =110:67:794,and the regression equation was obtained as follows: y=575.450-0.795x1+2.473x3-0.063x1x2,R = 0.987> 0.754,R2 is close to adj-R2,F value is 38.798> 9.300,which indicates that the regression equation is highly significant and the regression model is available.The results showed that xylanase had the strongest effect on the leaching of ferulic acid,and at the optimum ratio of compound enzyme the soluble amount of ferulic acid was 366.84 ?g/g,which was higher than that of uniform design,also the theoretical value was closed to 368.62?g /g,the program is feasible.(2)For the compound enzyme catalytic degradation basil stalk soak ferulic acid get on multiple regression analysis which take the analyzed by response surface model,obtained the model P = 0.0156 <0.0500,that indicated that the model had a significant effect.The optimum conditions obtained by Design Expert 8.0 software which as follows: the dosage of compound enzyme was 0.1029 g,the pH was 4.97,the enzymolysis temperature was 49.83 ?,the time of enzymolysis was 3.18 h,the predicted value was 369.75 ?g/g,the experimental value for 365.17 ?g/g,both close together,indicating that it is feasible to use this model to optimize the process.(3)The kinetics of the solution of reducing s?gar and ferulic acid catalyzed by compound enzyme was analyzed by particle diffusion model.The enzymatic hydrolysis process was divided into three stages.The two diffusion models were linearly fitted with a correlation coefficient of R2> 0.9800,the high linear correlation indicating that the model can be used to analyze the kinetics of dissolving the reducing s?gar and ferulic acid.(4)The heteropoly acid: HPA/TS-1,which had the best effect of degrading lignin,was selected by comparing the degradation efficiency of lignin with five different heteropoly acids.The range of each factor was obtained by single factor experiment heteropoly acid dosage 6-8%,catalyst volume 10-30 ml,the catalytic time is 70-90 min,catalytic temperature 160-180 ?,on the basis of this,the response surfacemethod was used to carry out multivariate regression analysis.The model P =0.0078<0.0100,indicating that the model had a significant effect.The optimum conditions obtained by Design Expert 8.0 software which as follows: the dosage of heteropoly acid was 7.04%,the volume of catalyst was 20.14 ml,the catalytic temperature was 172.45 ?,the catalytic time was 79.93 min,the predicted theoretical value was 31.69%,the experimental value was 32.30% closing to the theoretical value.