Identification And Genome Analysis Of Mucilaginibacter Pedocola Sp.nov.

In order to reveal microbial resources for heavy metal bioremediation,strain TBZ30^T was isolated from soil of a heavy-metal-contaminated paddy field.It is highly resistant to Zn²⁺（MIC = 3mM）and could remove zinc from solution.The strain produced abundant exopolysaccharides（EPS）which may be used to adsorb Zn²⁺.These feature makes strain TBZ30^T a great potential in heavy metal bioremediation.On the basis of 16 S rRNA gene phylogeny,strain TBZ30^T belonged to the genus Mucilaginibacter and appeared most closely related to Mucilaginibacter gynuensis YC7003 T,Mucilaginibacter litoreus BR-18 T and Mucilaginibacter mallensis MP1X4 T,the 16 S rRNA gene sequence similarities is 95.8%,95.4%,95.4% respectively.We hold classification and genome analysis to TBZ30^T.Cells of strain TBZ30^T were Gram-staining negative,rod-shaped,non-motile and non-spore-forming.The isolate was strictly aerobic,pink-pigmented,catalase-and oxidase-positive and produces exopolysaccharides.Strain TBZ30^T contained menaquinone-7 as the only ubiquinone.The main cellular fatty acids included summed feature 3,iso-C15:0,C16:0,iso-C17:0 3-OH and C16:1ω5c.The polar lipids were phosphatidylethanolamine,an unidentified phospholipid,two unidentified aminophospholipids,four unidentified aminolipids,three unidentified lipids and two unidentified glycolipids.The genomic G+C content is 49.0 mol%.On the basis of classification analyses,strain TBZ30^T represents a novel species of Mucilaginibacter,for which the name Mucilaginibacter pedocola sp.nov.is proposed.Here we describe the genome features of strain TBZ30^T,as well as the draft genome sequence and annotation.The genome size is 7,035,113 bp,contains 3,132 protein-coding genes（2,736 with predicted functions）,50 tRNA encoding genes,14 rRNA encoding genes and 54 heavy metal resistance genes.There were 21 zinc-resistance relevant genes and 11 EPS-production relevant genes in TBZ30^T,the genotype is quite consistent with the phenotype.This study enriched the diversity of genus Mucilaginibacter,supported more genome information of genus Mucilaginibacter,and provide a new way to Zn pollution bioremediation.