The Binding Capacity And Functional Properties Of Calcium-whey Protein Concentration Nanofibrils Polymers

At present,?-lactoglobulin,whey protein(WPI)as the material to produce fibrous polymer,to explore the sodium,calcium and other fiber-like polymer micro-morphology of the article more,However,there is little research about the amount of binding,the binding mechanism and the nature of the binding product to the whey protein(WPC-80)and calcium ion in the heat treatment time.This topic will focus on the WPC fiber polymer and calcium ion binding capacity of the discussion,the optimal conditions for calcium addition were obtained,the binding mechanism of WPC fiber and calcium ion was further analyzed by measuring the polymerization driving force(surface hydrophobicity,free thiol),thiocyanin-T fluorescence intensity and transmission electron microscopy,the functional properties of Ca-WPC fiber polymer were measured in finally,we hope this study can make some contribution to the field of application development WPC fiber polymer.The main findings are as follows:Optimization of WPC fiber polymer and calcium binding capacity The best addition of calcium ions: the calcium concentration in the solution was 16 mmol/L,heat treatment at 90 ?for three hours.Thioredin-T fluorescence intensity With the results of the test of thiampin-T fluorescence intensity,when the calcium concentration in the solution is 16 mmol/L,the Th T of Ca-WPC nanofiber polymer reached the minimum value at heat treatment for 3 h,the Th T of Ca-WPC particulate aggregates keep unchanged.Indicating that calcium ions and the ?-fold structure has been combined,the amount of calcium is better.Transmission electron microscopy observation WPC fiber polymers are completely different from the polymerized structure of WPC conventional polymers.When the calcium concentration is16 mmol/L,the calcium ion in Ca-WPC nanofiber polymer can be clearly observed at 3 h after heat treatment,which indicates that heat treatment can promote the binding of calcium ion to WPC fiber polymer.Functional properties By the determination of the foaming properties of the sample,it was found that both calcium and heat treatment can improve the foaming properties of Ca-WPC nanofiber polymers and reduce the foaming of Ca-WPC conventional polymers,the Ca-WPC nanofiber polymer had the highest foaming ability and foam stability when the calcium concentration was 16 mmol/L at 90 ? for 3 h,which was much higher than that of WPC nanofiber polymer.Through the results of emulsification,we can learn that both calcium and heat treatment can improve the emulsifying activity of Ca-WPC nanofiber polymer and Ca-WPC conventionalpolymer,Ca-WPC nanofiber polymer emulsion stability is higher than the control group,but its emulsifying activity is slightly lower than the control group.When the calcium concentration in the solutions reaches 16 mmol/L and heating three hours,the emulsifying activity and emulsifying stability of Ca-WPC nanofiber polymer reached the maximum at heat treatment at 90 ? for 3 h.According to the test results of gel hardness,we can learn that the calcium addition and heat treatment can improve the gel hardness of Ca-WPC nanofiber polymer and Ca-WPC conventional polymer.The gel hardness of Ca-WPC nanofiber polymer is higher than that of control group,when the calcium concentration in the solution reaches 16 mmol/L and heating three hours,the gel hardness of the WPC nanofiber polymer obtains the maximum value.According to the test results of gel elasticity,we can learn that the calcium addition and heat treatment can reduce the gel elasticity of Ca-WPC nanofiber polymer and improve the gel elasticity of Ca-WPC conventional polymer,when the calcium concentration in the solution reaches 16 mmol/L and heating three hours,the gel elasticity of Ca-WPC nanofiber polymer to a minimum value.According to the test results of gel viscosity,we can learn that the calcium addition and heat treatment can reduce the gel viscosity of Ca-WPC nanofiber polymer and improve the gel viscosity of Ca-WPC conventional polymer when the calcium concentration in the solution reaches 16mmol/L and heating three hours,the gel viscosity of Ca-WPC nanofiber polymer to a minimum value.In the above,when the calcium concentration was 16 mmol/L and heat treatment at 90 ? for3 h,Ca-WPC nanofiber polymer had the highest calcium binding capacity and its foaming property,emulsifying property and gel hardness were significantly improved.