Objective:To establish an ICP-OES method for the determination of Na+,Ca2+ and Mg2+ in peritoneal dialysis solution.The method will be validated through Total Error and be used in the sample stability study to evident this method is accuracy and reliable Method:The determination wavelength was selected from the characteristic wavelength of Na,Ca and Mg?Na279.553nm,Ca 393.366nm,Mg 589.592nm?.The parameter of Plasma Flow?12L/min?,Neb flow?0.65L/min?,RF Power?1400w?and View Distance were selected.The Y was the internal standard.The method was developed and validated with the concept of total error.The validated method was used in the stability study test.Results:Linearity of the method for Na is good in the range from 1992.5mg/mL to 2995.0mg/L?r=0.99998?.The total error was all within the 5%risk of Prediction Intervals at each concentration level over the range.Linearity of the method for Ca is good in the range from 56.1mg/mL to 84.0mg/L?r=0.9998?.The total error was all within the 5%risk of Prediction Intervals at each concentration level over the range.Linearity of the method for Mg is good in the range from 4.9075mg/mL to 7.3550 mg/L?r=0.9999?.The total error was all within the 5%risk of Prediction Intervals at each concentration level over the range.The sample stability tests for 6 months all met the criterias by using this test method.Conclusions:The method was proved to be accurate,sensitive,quick,simple,and reliable.It can be used for the determination of Na+ Ca2+ and Mg2+ in Peritoneal Dialysis Solution. |