Isolation Of Lead-and Cadmium-resistant Funges And Analysis The Effects In Promoting The Remediation Of Contaminated Soil With Phragmites Australis

To repair the heavy metal polluted soil, the combined restoration of plant with microorganism was used in this paper, which used the coexistence relationship of plants, soil and microorganism and that promoted the efficiency of the restoration of the heavy metal polluted soil. The study screened anti-fungal, lead and cadmium, to study its morphological, physiological and biochemical indices, and 18 SrDNA identification. Study on the adsorption properties and adsorption kinetics using reeds were pot experiment, the strain of heavy metal pollution and plant joint repairing effect.The microbial strains which can tolerate high concentrations of lead,cadmium are separated from the soil contaminated by heavy metals.Screen lead resistant strain-QD11, Screen cadmium resistant strain-QD09 and QD10. The results indicated that QD-10 selected can tolerate high concentrations of lead-cadmium.The MIC of three fungus are 2500mg/L(Pb2+),1000mg/L(Cd2+),1500mg/L(Pb2+)&1000mg/L(Cd2+)Growth temperature and pH value scope of three bacteria were broad through the physical experiment and had the ability of the hydrolysis of gelatin, starch and cellulose. The identification of the three stains were conducted through 18 srRNA, which showed that the NO.1 strain was Aspergillus, the NO.4 and NO.5 were Paecilomyces and the name of the strains were QD11, QD09 and QD10 respectively. Strain growth condition was optimized by orthogonal experiment and the results were as follows: biomass were increased by 81.18% than before optimization(QD11), 73.13%(QD09), 71.96%(QD10) respectively. Based on the adsorption research of the QD10, the best adsorption temperature of the strain is 30?; The adsorption rate reached 47.50%(Cd2+) and 47.50%(Pb2+) respectively when the pH is 6; When the biomass of bacteria was between 0.6-0.8, the adsorption effect was better; the adsorption rate increased within 60 min of the adsorption time while the adsorption rate tended to smooth after 60 min of the adsorption time and changed little. The isothermal equation was obtained by isothermal curve and dynamic curve and QD10 adsorption of living cells was in line with the Langmuir adsorption model by comparing the R2 and belonged to the monolayer adsorption. The analysis of heavy metals in morphology in soil of the potted plant experiment showed that the strain could not only reduce the heavy metal content in the soil, reduce the weak acid soluble state, but also square one and residue state of heavy metal, causing the oxidation state increase slightly. The amount of the heavy mental enrichment of the reed root after inoculate the strain was higher than that of the control group. The analysis of heavy metals in the body of reeds showed that the amount of the enrichment of the heavy mental in the soil of the underground parts was higher than that of the part on the ground and the ability of the enrichment of the heavy mental increased significantly through the inoculation of the strain.Heavy metal high tolerance strain was isolated from soil of Zhalong Nature Reserve, the pot experiment of strain and reed illustrated that the strain and reed could both decrease the amount of heavy metal in toxicity forms and the reed could enrich the heavy mental as well, which could decrease the toxicity of the heavy mental to the soil and make it an access to the prevention of heavy mental to the Zhalong Nature Reserve.