Flavonoids From The Branches And Leaves Of Illicium Wardii And Anti-bacterial Activity

Only one I. genus in the Illiciaceae family, which isshrubs or evergreen trees, is contained about 50 species in total in the world and mainly distributed in the north of the earth. While about 28 species and 2 variants of the I. genus in China discovered, most of them are distributed in the southwest, south and east of China, the 21 species of which are uniquely distributed in China.Illicium wardii A. C. Smith belongs to the Illicium genus of the Illiciaceae family.Although there exist many reports about the chemical constituents and bioactivity of Illicium genus, investigations on I.wardii were rarely reported, because of the limited distribution. The research focus on studying the continents from the branches and leaves of the I. wardii, which was obtained from the Gongshan county of Nujiang city, in Yunnan province. Detailed investigation on the branches and leaves of I.wardii led to the isolation of 15 compounds by different separated methods(silica gel column chromatography, prep.HPLCprep. TLC, Sephadex LH-20 column chromatography,, etc.), and their structures were identified on the basis of spectroscopic analysis(ESI-MS, NMR, IR etc.). The structures of these compounds were identified as 13 flavonoids and 2 other compounds. Among them,15 compounds were isolated from I.wardii for the first time.Fifteen compounds were tested for antimicrobial activity against ten bacterias using a microbroth dilution method. Only compounds ZY-4 showed moderate activity against the ten bacterias, respectively.