Study On The Nitrogen Removal Of Anaerobic Ammonia Oxidation In Oil Refinery Wastewater

In recent decades of wastewater treatment field, the treatment of refractory organic wastewater has become the focus and difficulty, and the oil refining wastewater is a kind of industrial wastewater which is difficult to deal with. The pretreatment-shortcut nitrification-anaerobic ammonia oxidation technology has a certain practical application value in treating oil refining wastewater, but there are few relevant researches at home and abroad. In this research, the effects of flow changes and backwashing on the denitrification performances of the embedded bioactive fillers and the bacteria blocks of the anaerobic ammonia oxidation mixed culture bacteria; the feasibility of using anaerobic ammonia oxidation-denitrification mixed culture bacteria treating oil refining wastewater were studied; the influences of the oil refining wastewater on denitrification capability of the mixed culture and bacterial community in the treatment process were investigated combined with the PCR-MPN and MiSeq high-throughput sequencing molecular biological techniques.The main achievements of this research are as follows: the mixed culture bacteria blocks were more sensitive to the changes of the flow than embedded biological activated carbon fillers; the removal loading and efficiency of NH4+-N, NO2--N and TN were increased obviously after the backwashing, but a frequent backwashing may lead to a damage of the formed micro environmental and a loss of newly grown bacteria in the embedded fillers; the anaerobic ammonia oxidation bacteria were more sensitive to the toxicity of the oil refining wastewater than the denitrifying bacteria in the domestication process; the coupling and synergistic mixed culture bacteria denitrification system could effectively resist the negative effects of the high COD concentration, high toxic substances of the oil refining wastewater to the physiological and biochemical process of anaerobic ammonia oxidation; the main effect on the anaerobic ammonia oxidation bacteria was their quantity denitrifying bacteria was their biochemical activity; the pollutants in the oil refining wastewater had great impacts on the total species, composition and diversity of the bacterial community, and the mixed culture bacteria evolved a certain tolerance to the toxic substances.