The Application And Investigation Of CuFe₂O₄/Co₃O₄ Nanoparticles Involved Chemiluminescence

Nanomaterial exhibits some excellent properties, such as small size effect,quantum size effect, surface effect, macroscopic quantum tunneling effect etc. due to its special structure.So, its catalytic properties, electrical properties, magnetic properties of nanomaterial are better than that of traditional materials. Chemiluminescence（CL） analysis has been greatly applied to analytical chemistry due to its features including simple optical systems requiring no light sources,high sensitivity,low detection limit, wide calibration range, and quick analysis.In recent years, it has been a more and hotter study topic about chemiluminescence catalyzed by nanomaterial.The first section of this dissertation is a review,which introduces the nanomaterial, chemiluminescence and the mechanisms and application of different kinds of nanomaterial involved to chemiluminescence in recent years.The application and mechanism of Cu Fe₂O₄ NPs involved Luminol-EDTA chemiluminescence system, Co₃O₄ NPs involved Luminol-H2O2 system, Co₃O₄ NPs involved Luminol-K3[Fe（CN）6] system were studied in the next three sections.CuFe₂O₄ is one of semiconductor and magnetic materials with band gap of 2ev. Cu Fe₂O₄ NPs have excellent physical, chemical properties, and peroxidase-like property which could catalyze hydrogen peroxide to produce hydroxyl radicals. Based on those, Cu Fe₂O₄ NPs involved luminol chemiluminescence was developed in this dissertation.Co₃O₄ nanomaterial is one of P-type semiconductor materials,which have peroxidase-like property. So, Co₃O₄ NPs involved luminol chemiluminescence was also developed.The new chemiluminescence system of Luminol-EDTA-Cu Fe₂O₄ NPs was firstly developed.The luminescence properties and possible luminescence mechanism were studied.It was found that rutin and Berberine hydrochloride could inhibit the CL of the Luminol-EDTA-Cu Fe₂O₄ NPs reaction.Combined with the flow injection technique, a new simple,rapid CL method was developed to detect the content of rutin in the rutin tablets and berberine hydrochloride in the berberine hydrochloride tablets.The possible mechanism of Luminol-EDTA-Cu Fe₂O₄ NPs was proposed by introduced UV-Vis spectra and chemiluminescence spectra.Under the optimal conditions,the relative CL intensity was linearly proportional to the logarithm of concentration（lgc） of rutin in the range of 2.00×10-8 2.00×10-5mol/Lwith a detection limit of 1.21×10-9 mol/L and the RSD of 2.54%. The new method has been successfully applied for the analysis of rutin tablets, giving values recovery in the range of 97¹02%.Under the optimal conditions,the relative CL intensity was linearly proportional to the logarithm of concentration（lgc） of Berberine hydrochloride in the range of 6.00×10-7².00×10-5mol/Lwith a detection limit of 1.64×10-8mol/L and the RSD 2.12%. The new method has been successfully applied for the analysis of Berberine hydrochloride tablets, giving values recovery in the range of 97¹01%.It was found that Co₃O₄ NPs could enhance the CL intensity of Luminol-H2O2 system. When terazosin hydrochloride introduced into the Luminol-H2O2-Co₃O₄ NPs system,the CL intensity would be greatly enhanced.Based on that,the CL system of Luminol-H2O2-Co₃O₄ NPs was firstly established to detect content of terazosin hydrochloride in the terazosin hydrochloride tablets with rapid,simple,wide linear range and high sensitivity.Under the optimal conditions, the relative CL intensity was linearly proportional to the logarithm of concentration（lgc） of rutin in the range of 4.00×10-8 8.00×10-6g/m L with a detection limit of 2.60×10-9g/m L and the RSD of 2.18%.The new method has been successfully applied for the analysis of terazosin hydrochloride tablets, giving values recovery in the range of 96¹03%.In addition, the possible mechanism of Luminol-EDTA-Cu Fe₂O₄ NPs was proposed by introduced UV-Vis spectra and chemiluminescence spectra.The Luminol-K₃[Fe（CN）₆]-Co₃O₄ NPs system was established. When Terbutaline Sulfate introduced into the system,the CL intensity would be enhanced. Based on that, the CL system of Luminol-K3[Fe（CN）6]-Co₃O₄ NPs was firstly established to detect content of Terbutaline Sulfate in the Terbutaline Sulfate tablets.Under the optimal conditions, the relative CL intensity was linearly proportional to the logarithm of concentration（lgc） of Terbutaline Sulfate in the range of 2.00×10-8 6.00×10-6g/m L with a detection limit of 4.20×10-9 g/m L and the RSD of 3.30%. The recovery in the range of 96 102.50%.In addition, the possible mechanism of Luminol-K3[Fe（CN）6]-Co₃O₄ NPs was proposed by introduced UV-Vis spectra and chemiluminescence spectra.