Electrochemical Determination Of Environmental Hormone Nonylphenol Based On Composite Film Modified Electrode

Nonylphenol(NP) is a main degradation product of nonylphenol ethoxylate(NPE) surfactants that are widely used in detergent, pesticide, paint, leather, textile, building materials, paper and other industries. Meanwhile, it is a kind of important fine chemical raw materials and intermediates, which called "sperm killer". Due to the mass use of NPE, it has been detected in wastewater and sludge in city. Compared with the NPE, NP is stable in the environment, that is, NP has stronger persistence and more bio-accumulation, which eventually passed to humans through the food chain continuously accumulated in the organism, and is harmful to the reproductive health of human and wildlife. Currently, NP has been recognized as a member of environmental endocrine disruptors(EEDs). Therefore, establishing an accurate, sensitive and rapid analysis method has very important significance in public health.Compared with other detection methods, electrochemical method has the advantanges of good selectivity, high sensitivity, low cost, rapid response, simple operation and without complex sample preparation process, etc.Nowdays, the electrochemical method based on modified electrode as the work electrode is one of the hot spots in the electrochemical research, so this paper focuses on the electrochemical detection of the composite film modified electrode as the workelectrode, and deeply discusses on the detection method of the environmental hormone nonylphenol, including the preparation ofcomposite film modified electrode, the analysis of electrochemical performance, the establishment and optimization of electrochemical analysis method and the analysis of environmental samples,the study contents are summarized as follows:(1) The study of electrochemical determination of environmental hormone nonylphenol based on thiol-β-cyclodextrin—graphene composite film modified gold electrodeIn this part, a novel electrochemical sensor based onthiol-β-cyclodextrin(β-CD-SH) and graphene(GR) hybrid-modified gold electrode(β-CD-SH-GR/Au) was prepared by electrochemical reduction of thiol-β-cyclodextrin-graphene oxide β-CD-SH-GO/AuThe composite of β-CD-SH/GO was directly synthesized on the surface of Au electrode by electrochemical polymerization using the chrono amperometry, and then reducted by cyclic voltammetry(CV). Meanwhile, the electrochemical behavior of nonylphenol(NP) was studied by CV and differential pulse voltammetry(DPV). Under the optimized conditions, the oxidation peak current exhibited a good linear relationship in the concentration range from 0.07 to 70 μ mol L-1(R=0.9952) and the detection limit was 0.061 μmol L-1(S/N=3). The prepared electrode was successfully applied to the determination of NP with the recovery from 92.36% to 105.6%, showing a practical prospect in real samples.(2) The study of glassy carbon electrode modified with gold nanoparticles/thiol-β-cyclodextrin—graphene for the determination of environmental hormone nonylphenolThe gold nanoparticles(AuNPs) was firstly polymerized on the surface of glassy carbon electrode(GCE) using the chrono amperometry, then thegold nanoparticles/thiol-β-cyclodextrin–graphene/GCE(AuNPs/SH-β-CD—GR/GCE) was directly synthesized with the complex of thiol-β-cyclodextrin(SH-β-CD) and graphene oxide(GO) by cyclic voltammetry(CV).Meanwhile, the electrochemical behavior of nonylphenol(NP) was studied by CV and differential pulse voltammetry(DPV). Under the optimized conditions, the oxidation peak current exhibited a good linear relationship in the concentration range from 0.05 to 50 μmol L-1(R=0.9901) and the detection limit was 0.017 μmol L-1(S/N=3). The prepared electrode was successfully applied to the determination of NP with the recovery from 96.11% to 105.9%, showing a practical prospect in real samples.