| The concentration distribution rule of amino acid in marine is an important indicator of marine carbon cycle and nitrogen cycle.The natural fluorescence of tryptophan（Trp）or tyrosine（Tyr）provides an effective tracing means and a convenient method for their detection-fluorescent spectrometry.The fluorescent property of fluorescence substances is not only related to the molecular structure,but also affected by environmental factors,such as temperature,hydrostatic pressure,solution acidity,etc.The two substances are coexisted in marine or other natural water,and the marine environment is complex and changeable.It makes sense to study the effect of typical environmental factors on the fluorescence of Trp-Tyr mixtures.Three-dimensional fluorescence spectrometry is a new efficient fluorescence analysis technology for its rich information,high selectivity and high sensitivity,and then it could be used to detect the fluorescence of mixed sys tem.Based on the theoretical knowledge and research progress about three-dimensional fluorescence spectrometry,in this paper,three-dimensional fluorescence spectroscopy was combined with parameterization method to study the effect of p H,heavy metal ion and hydrostatic pressure on the fluorescence of Trp-Tyr mixtures,54 groups of sample solutions were designed.The main conclusions are summarized here:（1）The spectral shape and fingerprint properties of Trp-Tyr in different pH are quite different.（2）Both Cu2+ and N i2+ have the strong ability to quench the fluorescence of Trp-Tyr,and the quenching become stronger as the concentrations of metal ions are higher.The quenching effect of Cu2+ on Trp-Tyr is stronger than Ni2+.（3）The fluorescent intensity of Trp-Tyr enhances with the increase of hydrostatic pressure,and the extents of the increase are related to the concentrations of metal ions.These results provide some reference value for the marine on-line detection of amino acid and the study of biological macromolecular structure. |
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