The Preparation And Application Research Of Biological Molecules And Light Response Poly(Carbonate)s Micelles

Aliphatic poly(carbonate)s are a kind of important biodegradable material, and as a kind of surface corrosion materials, it has good biocompatibility and physical mechanical properties, and it has many species. By changing the chemical structure of main chain and introducing side chain functional groups, it can make polymer material has a wide range of physical, chemical and biological properties to meet different needs. So it has been widely used in the biological tissue engineering and drug controlled release aspects. In recent years, with the continuous development of biomedical materials, the research of stimulus responsive polymer has involved in many chemistry fields, and has achieved certain results. This paper mainly focused on the preparation of stimuli responsive polymers, and studied the self-assembly behavior of the polymers in solution. On the basis of understanding the self-assembly behavior, we preliminary explored the potential applications of stimulus responsive polymer in detection. Specifically, the main contents are as follows:(1) With 2,2-bis(hydroxyl methyl)-propionic acid, propargyl bromide, ethyl chloroformate and monomethoxypoly(ethyleneglycol) (m-PEG) as raw materials, we synthesized ring carbonate monomer containing carbon carbon triple bond, 5-methyl-5-propargyloxycarbonyl-1,3-dioxan-2-one (MPC). The MPC were induced to ring opening polymerization by the macromolecular initiator m-PEG (molecular weight 5000) to get a kind of propargyl functional amphiphilic polymer PEG-b-poly (MPC). It was composed of hydrophilic side of PEG and hydrophobic of alkynyl functionalization, and can easily modify the hydrophobic by click reaction to make it have the function of specific response. We can get self-assembly micelles in the aqueous solution with the critical micelle concentration (CMC) of 11.6 μg/mL and the particle size of 70 nm around by the determination of scanning electron microscope (SEM), fluorescence spectrum and dynamic light scattering (DLS).(2) First through the click reaction, the above polymer PEG-b-poly (MPC) and the azide functional group of 2,4-dinitrobenzene sulfonyl compounds react to synthesize the amphiphilic polymer, PMPC-Dns, to modify hydrophobic polymer. The polymer micelles can respond the biological molecules selenocysteine (Sec) specially. And we test the Sec through packaging the fluorescent drug doxorubicin.Second, the PEG-b-poly (MPC) and the azide functional group of the selenium ether compounds react to synthesize the amphiphilic polymer, PMPC-2Se, to modify hydrophobic polymer. The polymer micelles can respond the biological molecules H2O2 specially.Finally, the PEG-b-poly (MPC) and the azide functional group of the nitrobenzyl compounds react to synthesize the amphiphilic polymer, PMPC-ONA, to modify hydrophobic polymer. The polymer micelles can respond the ultraviolet (UV) specially.Utilizing the means such as SEM, fluorescence spectrum and DLS, the self-assembly behavior and response characteristics of PMPC-Dns, PMPC-2Se, and PMPC-ONA are studied respectively.