Based On Nucleic Acid Probe And Nanomaterials Detection Of Biomarkers

Life science is one of the most important areas of science, it is closely related to human health and the development of the society.The attention human pay to their health and the environment is reached unprecedented heights.This also for workers in the field of scientific research put forward the higher request, prompting the researchers to further in-depth, refinement of life activities and related diseases to make more accurate judgment and interpretation.Method for early diagnosis of disease is mainly about some related to disease markers such as nucleic acids, proteins, enzymes, etc, for testing.And biological sensors for fast response, high sensitivity, the advantages of simple and convenient, attract more and more scientific research workers, are widely used in biomedical, environmental monitoring, food, medicine and other fields.In this paper, based on the current life science research hot spot and with the help of the function of nucleic acid and nano material advantages, we have developed two new type of biological sensing method for some biomarkers such as miRNA,dopamine,which is associated with diseases.In addition, the practicability of these methods are verified.MiRNA plays a very important role in the process of cell proliferation, differentiation, migration and apoptosis.Accurate analysis the content of miRNA for better understand their role in cancer and cancer treatment is crucial.In Chapter 2,We use poly?A? polymerase to stretch out a poly ?A?-tail in the end of the target,the tail which could combined with coralyne and then formed a double chain.Then join the SYBR Green I ?SG I?.SG embedded double-stranded nucleic acid, whose fluorescence signal enhanced significantly.At that,we have developed a simple and sensitive, low cost and label-free sensing methods for inspection of miRNA.Utilize ucleic acid dye SYBR Green I as the fluorescent indicator, without prior to fluorescent tags, in addition,take advantage of magnetic beads,that has good separation and enrichment characteristics, to further improve the sensitivity and selectivity of analysis method.By changing the the sequence of biotin capture probe, the method can be further applied to other nucleic acids detection.This is expected to be for nucleic acid detection as well as the related biomedical research to provides a simple, convenient and economic analysis of the test platform.Dopamine,an important neurotransmitter of endocrine,regulates the body exercise and mental activities of the central nervous system.Dopamine concentration change is closely related to many diseases.In Chapter 3,Due to the excellent properties of g-C₃N₄,we set up a kind of lable-free, "turn off" type test of dopamine.The fluorescence of g-C₃N₄ was quenche after captured target, by the size of the fluorescent value so as to learn the target concentration of dopamine. Compared with other test methods,the method puts forward in chapter 3 is higher sensitivity, simple operation, expected to further reduce the related disease clinical diagnosis economy and time cost, improve the treatment efficiency.