Preparation And Application Of Biosensors Based On Nanomaterials

Since its discovery,biosensors have been widely used in bioengineering,food safety inspection,environmental monitoring and medical diagnosis due to their good biocompatibility.However,with the increasing requirements for sensors,traditional biosensors are unable to meet the current detection needs in terms of time,cost,signal sensitivity and detection line due to their own limitations.The emergence of nanomaterials provides a solution to the problems encountered by biosensors.Nanomaterials have superior electrical,chemical and biological properties.In particular,it has a broad application prospect in biologies,such as the size of nanomaterials and biochemical molecules,good biocompatibility and other advantages,as well as as the conduction of biosensors can provide a good basis for new biosensors.This paper is divided into the two parts:?1?A fast and sensitive method for detecting dopamine was designed.Fe₃O₄ nanoparticles were prepared by co-precipitation method,and the surface modification was used to synthesize Fe₃O₄@Au@Cysteine.Then a solution of Fe₃O₄@Au@ Cysteine and Tyrosinase was further fixed to a forked finger electrode.Tyrosinase catalyzed dopamine detection.Local concentration aggregation can be achieved by the action of iron tetroxide in the magnetic field and the formation of magnetic nanowires on the fork-finger electrode to improve the signal sensitivity.?2?Study on artificial enzyme construction of sensor.PCN222,a Metal Organic framework material?Metal Organic Frameworks,MOFs?,was synthesized and characterized by electron microscopy and spectrum.Because of its high conductivity and special pore structure,PCN222 was applied to construct chitosan-PCN222 /GC sensor in three-electrode system.In addition,cyclic voltammetry and timing current method were used to characterize the sensor and to detect copper ions and catechol rapidly,which provided an effective method for the detection of copper ions and catechol.