Synthesis And Performance Analysis Of Magnetic Molecular Imprinted Microspheres

The magnetic molecular imprinted microspheres (MMIMs), which combinine magnetic particles to molecular imprinting, have many advantages, such as easily separation from solution and high selectivity for the compounds having similiar structure and properties. Now there were many reports on preparation and application of MMIMs, but they were rarely used in amino acid separation. Compared with the traditional separation methods, the separation vector was given high selectivity and magnetic response. The separation of MMIMs not only can be used for continuous production, but also improves the separation selectivity, which will provide a new technology roadmap to the separation of amino acids. In this study, we have attempted to use MMIMs to separate phenylalanine and tyrosine, which gave a good separation result with the separation factor a of 2.269.In this study, some NMIMs were prepared via dispersion polymerization, emulsion polymerization and miniemulsion polymerization. The physical and chemical properties of the polymers were analyzed by scanning electron microscope, thermo gravimetric analyzer, particle size analysis and IR.Fe3O4 magnetic nanoparticles were prepared via coprecipitation method and modificated with oleic acid. Single oleic acid/Fe3O4 magnetic nanoparticle and double oleic acid/Fe3O4 magnetic nanoparticle were prepared and its stability, appearance and magnetic were characterized.Magnetic polymer microspheres were prepared by encapsulating oleic acid/Fe3O4 particles with MNIMs. Its Ms is 30.43 emμ/g.Phenylalanine as template molecule, oleic acid/Fe3O4 particles as magnetic carriers, St and MAA as monomer, PVP as dispersant,AIBN as initiator, the Phe-MMIMs was successfully prepared on the basis of the above experiments. The results showed that the MMIMs had a good affinity and recognition with the template. Its saturated adsorptive capacity was 86.12μmol/g. Phe-MMIMs have a high selective adsorption for phenylalanine. Comparative with tyrosine, the separation factor is 2.269 and comparative with MNIMs, the separation factor is 2.283.