Synthesis And Characterization Of Polyethylene Glycol And Peptide Side Chains For Amphiphilic Polyphosphazene

Amphiphilic polymer as a drug carrier of recent studies,can self-assemble in aqueous solution to form a polymer micelle,a higher drug loading capacity of its core,and has a unique distribution in the body.This study synthesize a series of polyethylene glycol-amphiphilic graft peptide polyphosphazene,which in an aqueous solution are capable of forming micelles by self-assembly,the micelle has a unique core-shell structure,and It can stably exist in water.Amphiphilic Polyphosphazenes micelle with structural stability,size controllable can escape phagocytic reticuloendothelial system(RES)to identify and enhance penetration retention effect(EPR effect),embedding hydrophobic drugs by passive targeting to tumor tissue delivery of drugs to improve the therapeutic effect.The chemical structures of series of the amphiphilic graft polyphosphazenes were characterized by using nuclear magnetic resonance spectroscopy(~1H-NMR),infrared spectroscopy(FT-IR),and Ultraviolet spectrum(UV)method.Studying amphiphilic Polyphosphazenes’ self-assembly in aqueous solution by utilizing pyrene fluorescence probe method.CMC curves were plotted according to fluorescence intensity measured.The critical micelle concentration of measured synthetic PEG/EtGFLGK-PPP-1,PEG/EtGFLGK-PPP-2 and PEG/EtGFLGK-PPP-3 was 0.0265g/L,0.0189g/L and 0.0103g/L.The CMC of an amphiphilic polymer was related to the ratio of the size of the hydrophobic segment,when the increase in the proportion of the hydrophobic segment,CMC will be reduced accordingly.Dynamic light scattering(DLS)were used to study the amphiphilic graft polyphosphazene in aqueous solution with the particle size and distribution.The average particle size of the three different proportions of hydrophilic and hydrophobic amphiphilic Polyphosphazenes were in the range of 10-20nm.