Bio-effects Induced By Electromagnetic Radiation In Mesenchymal Stem Cells And Neural-like Cells

Nowadays,a variety of wireless communication equipments are widely used and draw much concern to their EMR bio-effects.Because of the difference of cell sensitivity and weakness of the electromagnetic field,their bio-effects are hard to capture,thus there is no conclusive conclusion.Studies suggested that stem cells are sensitive to environment and applicable used to detect the bio-effects of EMR.Anthropogenic stem cells may be one of the best cell models to detect the effects of EMR exposure.As for EMR from mobile phone,our brain may be the most closest organ to the phone and might be influenced.Because EMR is weak and its effect is difficult to detect,it is necessary to observe its long-term exposure effects.But anthropogenic NLC which can be continuously passaged and used for long-term exposure is rare.Hence,our main research is as follows:?1?Construction of a NLC line that can be continuously subcultured for long-term EMR exposure.Human umbilical cord derived mesenchymal stem cells were induced to differentiate into NLC with bFGF as pre-inducer followed by BHA,DMSO and?-mercaptoethanol as inducers.The NLC were identified by morphology and expression of Nestin,NSE,GFAP and MAP2.The results showed that the induced cells had protrusions and were positive of Nestin,NSE and GFAP by immunofluorescence,which means MSCs were turn into NLC.Nestin expression at50^thsubculture was lower than 11^thsubculture,and NSE expression at 50^thh subculture was higher than 11^thh subculture,which indicated that nerve stem cells have differentiated into NLC.?2?Study on the effects of short term exposure of MSCs and NLC to EMR.The oxidative stress,which has its own balance,responses rapidly after stimulated by environment.ROS is a common indicator which might damage cellular macromolecules and cell apoptosis.Changes of introcellular ROS after exposure were observed by flow cytometry with DCFH-DA fluorescence probe.Annexin V was used to detect the externalization of PS during early apoptosis.PI was used to label nucleus in apoptotic or necrotic cells.Lipid peroxidation was measured by colorimetric method with TBA and MDA.The results as follow:?ROS positive percentage in MSCs and NLC were increased after exposure,suggesting that EMR might cause oxidative damage in MSC and NLC.?The early apoptosis rate of MSC were higher than sham exposure group,and the late apoptosis rate increased significantly at 5 h.The early apoptosis and late apoptosis rate of NLC was not significant,and the necrosis rate increased significantly at 3 h and 5 h.?There is no significant difference in MDA between exposure group and sham exposure group.?3?Detection of cell proliferation and transformation capacity after 60-day exposure in MSCs and NLC.The results showed that the proliferation capacity of MSCs was significantly increased after 60 days exposure.Cell plate cloning showed that there is no clone formd in MSCs,however,the number of NLC clones was significantly improved compare to the sham exposure group.No soft agar clones formd of the two cell lines,either in the exposure group or in the sham exposure group.In addition,based on our previous studies,we tested the expression of radiation-sensitive genes p53 and Serpinb5.The expression of p53 was increased 3.26times after exposure and the Serpinb5 decreased slightly in MSCs;and these two genes were slightly lower in NLC compared to the sham exposure group.The expression levels of p53 and Serpinb5,which were related to cell injury and post-injury repair,should be followed dynamically,and their corresponding signal pathways need to be reseached later.