The Basic Characteristics Of Microorganism And The Mechanism Of Cu Uptake And Transformation In Cu Mining Area

With the development of all walks of life in China,the demand for Cu mineral resources is increasing.The search for concealed deposits also requires the introduction and development of new prospecting technologies to achieve multi-disciplinary cross-cutting,so as to increase the chance of successful prospecting.Microbiological prospecting has brought a new turning point with the development of contemporary biology,and has attracted the interest of scientific researchers with its green and environmental advantages.The combination of chemical analysis methods for heavy metal elements in microorganisms and modern biology provides theoretical data for the interaction between microorganisms and soil heavy metals,providing new ideas and complementary methods for prospecting strategies.In this paper,the basic traits of the bacteria were studied by cultivating the protobacteria in the soil of the Yingpan Jinman Copper Mine and the Chimashan Copper Mine in Yunnan Province.The correlation between the Cu content in the bacteria and the Cu effective state in the soil was analyzed.The mechanism of absorption and transformation of copper by specific bacteria was explored.The study found that:?1?Cu content in microorganisms collected from the soil of copper mining areas was positively correlated with the concentration of weak acid extracted Cu in the soil of the F1 state,indicating that the specific microorganisms found in typical copper mining areas have Cu in soil.Indicates the role.The identification results showed that the bacteria were Rhizobium/Agrobacterium and Acinetobacter.To a certain extent,these two kinds of bacteria were indicative of Cu in the mining area,showing the potential for prospecting applications.?2?The specific microorganisms found in this study not only have potential indications,but also have certain Cu tolerance.Through the addition of organic Cu?Ac?₂ and inorganic CuSO₄microbiological tolerance tests,it was found that the tolerance of copper to Rhizobium/Agrobacterium and Microbacterium can reach 200 ppm.?3?The microbial diversity in a typical Cu mining area is affected by the Cu content in the soil.Soil F1 state weak acid extraction state>6000 ppm,only tolerant microbe present;F1 state>2000 ppm can be found Rhizobium/Agrobacterium and resistant Acinetobacter exist,and when F 1 state<2000 ppm,biological Diversity has increased significantly.?4?The specific microorganisms found in the typical copper mining area of this study have the function of absorbing and transforming Cu.X-ray absorption spectrum analysis showed that Cu was present in the soil as inorganic CuCO₃,Cu₃?PO₄?₂,Cu₂S;as the form of Cu-S,Cu-HGAS in microorganisms.The result shows that when Agrobacterium has absorbed Cu,Cu is converted into Cu-S,Cu-HGAS?copper-polygalacturonic acid?in microorganisms.Since the root system of plants in the soil secretes organic acids,the carbonate-bound Cu is activated,and after entering the microorganism,it combines with S to form CuS precipitates,forming a detoxification mechanism for microorganisms.When reduced oxidizing bacteria presents or Eh changed in the soil,Cu₂S is oxidized to Cu²⁺,which is absorbed by microorganisms and forms a Cu-HGAS complex with HGAS?galacturonic acid?.However,the exact location is still unclear and further research is needed.