| In our previous work to investigate free radicals distribution in a living animal,we found that free radicals could be allocated specifically in connective tissues such as linea abla when SD rat was intravenously injected with DCFH-DA,an free radicals indicator.The further confirmation of the above mentioned discovery was carried out by visualizing free radicals distribution in New Zealand rabbit and cynomolgus macaques using DCFH-DA and mito-SOXTW Red injection.A C57BL/6-TgH(mito-cpYFP)transgenic mouse was constructed,in which superoxide-specific cpYFP was expressed in mitochondria to investigate the patterned free radicals distribution and its relation to mitochondrial free radicals and conduction.The major findings are as the following:1.Free radicals were identified in the connective tissues of both New Zealand rabbit and cynomolgus macaques,and the fluorescent light was identified to emit from collagen fibers,indicating that free radicals may transmit along collagen fiber.2.In C57BL/6-TgH(mito-cpYFP)mice,fluorescent light appeared in below the belly button after birth and extended upward gradually along the linea alba with days,and the fluorescent intensity peaked at 11:30-13:30 everyday.3.When electric current flowing through the linea alba the free radicals fluorescent light appeared.The linea alba current was 0.7 ?A for wild type C57BL/6 mice while it was 0.1 ?A for the transgenic mice 70 minutes after the wiring,and reached 0.4 ?A when hydrogen peroxide was added to the liver surface to generate oxidative stress.The results suggest that free radicals transductions along the linea alba occurred in the form of electric current which is related to mitochondrial metabolism.Those results confirm the regular distribution of free radicals and transduction in the form of electron in the connective tissues of mammals,implying the existence of a system consisting of the connective tissues for the collection,storage,transduction and disposal of mitochondrial leak electron. |
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