Comparative Research Of Life History Between Chingia Ferox And Phyllitis Japonica

In this paper,select the two representative species,one is thelypteridaceae which since its establishment the families belong to the problem has been controversial,and the other is aspleniaceae which one of the largest groups of pteridophytes,Chingia ferox(BL.)Holttum and Phyllitis japonica Kom as experiment material,using hydroponics and soil culture and Knop 's solid medium three methods,through artificial cultivation and comprehensive comparative observation of the life cycle of both development and artificial sex seedling,reveal their stable rules of reproductive biology,to explore ferns system evolution accumulated lots of basic information;By comparing the morphological diversity,discussing the reproductive biology systematics of basis.In terms of sexual generation,through hydroponics,soil culture and Knop 's methods of solid culture medium for culture and observation,selection typical materials to Made a temporary water tank,and observed under optical microscope(OLYMPUSBX53)and take pictures,and uses the method of paraffin to observation and comparative study the continuous slice of the two reproductive organs and embryo.Experimental results show that the Chingia ferox and Phyllitis japonica has the rich diversity and stability of regularity in the spore germination,protonema development,trichomes characteristics,the formation of the growing point,the development of rhizoid and so on;in terms of reproductive organs,this article found that the growth of archegonium of Chingia ferox and Phyllitis japonica are similar,can be summarized as the archegonium initially by the original on the surface of the leaf surface develops from a primitive cells,primitive cells periclinal division,forming the primary cells,the central cell and abdominal wall primitive cells,primary cells split to form cervical cells and parietal cells,the central cell division into primary ditch cells and primary stomach cells,primary split into two neck ditch ditch cells,primary ventral cell divide into the ventral canal cell and egg cells,abdominal wall primitive cells divide into Abdominal wall cells.the spermatangium of Chingia ferox and Phyllitis japonica is also similar,are composed of basal cell,choanocyte and cover cell.So far,the aspect of embryonic development of Chingia ferox and Phyllitis japonica has not been reported,so in this article,through artificial mass culture two kinds of ferns prothallus Serial section by using paraffin section method,and observedthe characteristics of the two ferns embryonic development: the embryonic development of Chingia ferox and Phyllitis japonica almost same,zygote division for the first time along the X,to form two daughter cells,again divided along the Y and Z,globular embryo formation of eight cells,continue to split appeared first leaf and the first root apical cell respectively.In vegetative organs,this article by semi-thin sectioning observed two fern root,leaf,petiole,and the porosity,the scales are carried on the detailed observation,for system classification of taxa,provides the basis of detailed data and related research.In addition,based on the detailed observation of paraffin sectioning Chingia ferox sporangia of developmental process,Chingia ferox sori borne grouped in spore both sides of the leaf veins,Sporangium circular,without indusium,Chingia ferox sporangia development belongs to a common type of thin capsule fern,only by a layer of cells,cystic wall thin,the suspensor but only two columns of cells,when sporangium differentiation is complete,sporangium differentiation is mainly composed of three parts: the sporangium capsule wall,inner and outer tapetum and spore cells,when spores mature,only capsule wall and the outer tapetum.At the same time it also in different plant transplanting,rejuvenation,finally to colonize of Chingia ferox's young spore plants.Based on the above research results,this paper discusses the sexual generation,the pattern of embryonic development and its significance in systematics,to study the reproductive regularity of two families and the system evolution relationship provides evidence,also learned from the angle of microscopic origin and initial development of a plant in different habitats to study vascular plants the system origin of root,stem and leaf sequence systematics basis was provided for and their mutual relations,but also to Chingia ferox powerful long-span environment adaptability provide embryological evidence and successfully tried artificial sex and seedling,mass propagation Chingia ferox and Phyllitis japonica,for China's development of Chingia ferox and Phyllitis japonica outside gardening to provide technical support.