Research Of Cell Biology On Phaneroplasmodia Of Sevaral Myxomycetes Of Physarum

Plasmodia of the non-cell myxomycctes is the vegetative growth phase in its life cycle.The materials used in this experiment are contain Physarum polycephalum which belong to Physarales,Physaraceae,Physarum;Fuligo septica which belongs to Physarales,Physaraceae,Fuligo;Physarella oblonga which belong to Physarales,Physaraceae,Physarella.These three kinds of plasmodium are all phaneroplasmodia which is convenient to culture and observe it.These three kinds of plasmodium were feed and cultured for a long time.Physarella oblonga and Fuligo septica were cultured in the 2% water-agar mediun and fed with sterile water and oat powder;Physarum polycephalum has been cultured in substrate of MSD.Microscopic photography camera technology and tissue section technique were used to research three plasmodia.1,the rusult of observation showed that the structure of the vein of mature well-growing plasmodia of Physarum polycephalum is relatively thick,the fan-shaped structure is thicker,branches are not obvious and the color is yellow.2,When the plasmodia of Physarella oblonga grows well,the vein is thick,fan-shaped structure is thick,branched significantly,the color is more yellow and is deeper than the plasmodia of Fuligo septica,like yellow orange.3,well-growing plasmodia of Fuligo septica,fan-shaped structure is clear,the vein are relatively weak compared to the Physarella oblonga,branches are clear and has a bright yellow color.The growth conditions of the plasmodia of the Physarella oblonga and Fuligo septica are relatively broad,and the growth conditions of the plasmodia of the Physarum polycephalum are more strict.The experiment has through normal production,Leica CM1950 frozen sections and paraffin section for the plasmodia of Physarella oblonga and Fuligo septica,and apply conventional staining,Hoechst 33258 nuclear fluorescent dye staining,ActinRed staining for observation.The results shows that there are a large number of nuclei in the plasmodia,which can lay the foundation for the accurate calculation of the number of nuclei according to the distribution of the nuclei in the specific size range,and explore the functional volume of the nucleus on the cytoplasm.The protoplasmic flow in the phaneroplasmodia is a model material to study of cyclosis.The vein of phaneroplasmodia consist of the gel-like ectoplasm and the inner more fluid endoplasm.The fluorescent probe shows that actin exist in the gel and sol junction,a large number of microfilaments together to form microfilament,indicating that actin and as skeleton to supported the vein of plasmodia.Sampled the fresh plasmodia of Physarella oblonga and Fuligo septica every 20 minites,observe the nuclear cycle of synchronous mitosis and find that the nuclear present periodic changes,the shrinkage and dispersion of chromosomes in the nuclea.It can be deduced from the observation that the period of nuclear synchronous mitosis of Fuligo septica is about 9 hours,the period of Physarella oblonga is about 7 hours.The unique biological characteristic and the characteristics of the nuclear exist in the plasmodia of myxomycctes make it a good model material for cell biology research and the signigicance of to study this organism.