Transcriptome Analysis Of Deep-sea Hydrothermal Vent Shrimp Rimicaris Exoculata And Study On Copper-zinc Superoxide Dismutase

Deep-sea hydrothermal vents are the unique ecosystem,where sunshine can not reach and are absolutely dark.Thereby,instead of photosynthesis,organisms use chemosynthesis for primary production.Except that,high temperature,acidity,high pressure,reducibility,lots of dissolved heavy metal(aluminum,copper,cobalt,iron,zinc,magnesium,plumbum)and scarce food source also make the deep-sea hydrothermal vents extreme harsh.Surprisingly,lives here that can adapt to the extreme environment are abundance,and attract the scientist interest.R.exoculata is one of the dominant species around Atlantic hydrothermal vent.Over 3000 shrimps per square metre had been repored.R.exoculata is one of the dominant species around Atlantic hydrothermal vent.Over 3000 shrimps per square metre had been repored.In this study,R.exoculata was collect from Atlantic hydrothermal vent(3059 m in depth,15°9'S,13°21' W).In order to understand the metabolism characteristics,information of the function genes and the mechanism of adaption to hydrothermal vent,Illumina 2000 platform was used for transcriptome sequencing.Totally,4,887,093,420 nt raw reads date,assemble into 35772 uingenes were obtained.Then 20450 predicted protein coding genes were determined,and 15660,6230,13575,11783,6321,8142 genes were annotation by NR,NT,Swiss-Prot,KEGG,COG,GO database,respectively.By analyzing transcriptome of R.exoculata,we learn the overviews of its molecular feature.In the basal metabolism,we found valine,leucine,isoleucine biosynthesis pathway intact.It suggested R.exoculata might have the ability to synthesis these essential amino-acid by itself.Then we analyzed the R.exoculata environment adaptation correlation pathway especially sulfur metabolism.Abundant genes were annotated as sulfide detoxification pathaway in transcriptome of R.exoculata.which can catalyze sulfide hydrogen transform into elemental sulfur or organic sulfur to reduce the toxicity.By the mean time,we consider it is also probably exist SOX system(which play a important role in sulfur circulation)in R.exoculata.We also chose the Toll-like,JAK-STAT and IMD pathway as the representative for R.exoculata immune pathway analysis.There is integrality in Tolllike,JAK-STAT pathway,and incompleteble in IMD pathway.Cause the environment pressure are different.Redox reaction usually produces reactive oxygen species(ROS)in organisms.ROS will make lipid peroxidation,base mutation,DNA chain breakage,protein injury,.Superoxide dismutase(SOD)plays an important role in oxidation resistance.It can make superoxide ion disproportionate translate into H2O2,then further be translated into water and oxygen by catalase.It exists in almost plants,animals,microbes.And widely used in medicine,food industry,agriculture,cosmetic and so on.In this study,we cloned and expressed an R.exoculata SOD gene in Escherichia.coli,named RESOD.After purification and renaturaion,RESOD displayed a highest activity at 10?,pH=5.And cobalt,magnesium,manganese,barium,calcium could inhibit RESOD.The results suggested that RESOD is a alkali resistance and low temperature enzyme that may has potential value in application.