Comparative Morphology Of Important Diagnosis Characters Of Leptomias Generic Group From China

The pronotum,metendosternite and genitalia of weevils are concerned with movement and reproductive.Metepisternum is a very important structure in the genus classification of Leptomias generic group.In this paper we selected 20 species of Leptomias generic group as the materials for traditional description and geometric morphometric analysis.We took 433 photos.We used Tps series software to do quantification of morphological characteristics,used MorphoJ 1.06 c to do principal component analysis and canonical variate analysis,used NTsys 2.10 e to create the phonetic tree.Through all above methods,we studied the difference significant of pronotum,metepisternum,metendosternite,male and female genitalia between intergeneric,interspecific and intraspecific.We also study the difference significant between male and female,and the canonical variate analysis of shape and elevation.The research results:1 standard length: 4.92–10.60mm;elytral length-width ratio: 1.27–1.71;pronotum length-width ratio: 0.71–0.99.2 all of the species' pronotum with scales carvelsystem and separate from each other,except Geotragus himalayanus and Xizanomias altus.The pronotum of L.schoenherri,L.micans,L.aeneus,L.tuberculatus,H.hengduanensis and T.rotundus on both sides covered with pinnate scales.3 anterior tendon is located in 1/3–1/2 of the arm;and all of the species' anterior part of longitudinal flange are less than longitudinal flange,except L.aeneus?L.tuberculatus and H.guinanensis;stalk and arm ratio between 0.25–0.33;the angle between median keel and arm is 40°–75°.4 male genitalia: penis circumflexion of L.yulongshanensis is the most small one,and H.guinanensis is the most big one;the apex of penis is divided into round and truncated;penis is divided into bent downwards,bent upwards and straight at apex.5 female genitalia: bursal sclerites are different in shape;the angle between colum and ramus is 30°–60° or ?90°;ramus is trapezoidal,different in lengths or disappear(like L.qomolangmaensis).6 analysis of morphological difference significant between the male and female: the P-values of pronotum from permutation tests(10000 permutation rounds)for Mahalanobis distances among male and female are all greater than 0.05,except L.acutus,L.yulongshanensis and T.trianguloplatus;it illustrates that male and female are not significantly different in most species.7 analysis of morphological difference significant for interspecies and intergenus: the P-values of pronotum,metepisternum and metendosternite from permutation tests(10000 permutation rounds)for Mahalanobis distances among intraspecies are all equal 1,but among interspecies are all less than 0.05 and among intergeneric only in part are less than 0.05;it illustrates that interspecies is significantly different,and intergeneric is not significant difference.8 we regard 600 ~ 1000 m,2000 ~ 3000 m,3000 ~ 4000 m and 4000 ~ 5000 m as a condition of classification to do canonical variate analysis about pronotum and metendosternite;the P-values of pronotum and metendosternite from permutation tests(10000 permutation rounds)for Mahalanobis distances among groups are all less than 0.05;it illustrates that pronotum and metendosternite have some relevance with species distribution of the altitude range.