The Effect Of Macrobrachium Nipponense And Chinensis Cipangopaludina Density On Bacterial Community Diversity On Biofilm

In this paper, Biolog-eco and high-throughput sequencing methods were used to research the metabolism and community structure of bacterial communities on biofilm which applied in aquaculture or ecological restoration. In the condition of not feeding, the effect of Macrobrachium. nipponense and Cipangopaludina chinensis density on biofilm bacteria diversity and aquaculture water quality were analyzed.The change of water quality in M. nipponense experiments. The total nitrogen, ammonia nitrogen, total phosphorus content were obviously higher than the control group, and with the density of M. nipponense, the total nitrogen, total phosphorus, ammonia nitrogen also increased.The change of water quality in the C. chinensis experiments. The change of ammonia nitrogen and total nitrogen showed the increasing trend. The addition of C. chinensis had a certain role on the removal of total phosphorus, the densities of 400ind/m3 experimental groups had a better removal effect.Effect of M. nipponense density on the bacteria's metabolism and diversity on biofilm. The AWCD curve showed that the slope in the 12-48 h was maximum, changed slowly in the 48-96 h, and stabilized after 96 h. The AWCD value of the control group was less than that of other experimental groups over time. All kinds of carbon sources were used in the early stage and the end stage of the experiment, the alcohols, amines were utilized less in the mid of the experiment. The change of diversity index showed a gradual increase over time, the biodiversity of control group was less than that of test groups.In the M. nipponense experimental group, the bacteria on biofilm were belonged to 18 phylum, 115 classes, 191 orders, 270 families, 402 geniuses. There were some unknown groups in each taxon. At the phylum level, the main advantage phylum was Proteobacteria. In the genus level, the Paracoccus and Aeromonas were the main advantages genus in M. nipponense experimental group.Effect of C. chinesis density on the bacteria's metabolism and diversity which on biofilm. The AWCD curve showed that the slope in the 12-48 h was maximum, in the 48-96 h was slow, andwas stabilized after 96 h. The AWCD value of the control group was less than that of other experimental groups over time. All kinds of carbon sources were used in the early stage of the experiment, and then the alcohol and acids carbon resourcesutilization reduced, the amine carbon source utilization reduced in the end of the experiment. The change of diversity index showed a gradual increase over time, and the boidiversity of control group was less than test groups.In the C.hinesis experimental group, the bacteria on biofilm were belonged to 18 phylum, 116 classes, 190 orders, 269 families and 396 geniuses. There were some unknown groups in each taxon. At the phylum level, The main advantage phylum was Proteobacteria. In the genus level, the Paracoccus was the main advantages genus.