Analysis Of Siniperca Chuatsi Slow Muscle Transcriptome And Skeletal Rhythmic

Biological clock is the activity of the body's internal rhythm that can make the organism to maintain a constant change, even if the external conditions change. The organism biological rhythm is mainly controlled by the hypothalamus, but the peripheral tissues also have circadian rhythms, such as muscle. Fish muscle fiber types are similar to those of human that contain the fast muscle and slow muscle which was mainly responsible for the metabolism and maintained oxygen movement, respectively. In fish fast and slow muscle is quite distinctive and easy separation while in mammals it is embedded and distribution which scattered and difficult to separate. At present, there are few studies on muscle biological clock. This paper screened 15 clock genes (arntl1?arntl2? clock?cry1?cry2?cry3?cry-dash?npas2?nr1d1?nr1d2?per1?per2?per3? Rora and tim) from Chinese Perch red muscle transcriptome, and all clock gene made phylogenetic tree found that they were closely related to their orthologs in other teleost fish. These results proved transcriptome library clock gene sequence and function prediction accuracy. In the fast muscle, the clock genes arntll, arntl2, clock, cry1, nr1d1, nr1d2, per1, per2, per3 and Rora, functional genes mbnll, mrf4, mstn, myoD, myoG, myf5 and pcna had daily rhythmicity. There is a positive and negative correlation between the rhythms of clock genes. The pcna showed moderate negative correlation with arntl2, cry1 and per1. The mbnll positively correlated strong with the components of the the daily expression of arntl1, cry1, nr1d1, per1, per3 and clock. The myoG also strong positively correlated with arntl1 and per1. In the slow muscle, the clock gene arntll, arntl2, clock, cry1, cry3, npas2, nr1d1, per1, per2. Rora and tim, functional genes including foxk2, mrf4, mstn, myf5 and pcna displayed daily rhythmic expression. Rhythm of clock genes only had positive correlation. Transcript levels of pcna positively correlated moderate with the components of arntl2, cry1 and per1. The myf5, mrf4, pcna and mstn in the slow muscle showed strong correlation with cry3 and npas2. The functional gene foxk2 also showed strong correlation with the components of the transcriptional activator arm arntl2. The results showed that, regardless of the fast or slow muscle, there was a strong correlation between multiple genes. These clock and functional genes firstly co-ordinate and antagonize its function, then regulate Chinese Perch muscle fiber growth, metabolism and the biological clock. The above results also provided the basis for further study of the relationship between them.