The Functions And Regulations Of Long Non-coding RNA Lnc400 In Neural Precursor Cells

Long-non coding RNAs (LncRNAs) are non-protein coding transcripts longer than 200 nucleotides transcribed by RNA polymerase ?, which show a wide range of functions in epigenetic, cell cycle and cellular differentiation and nerve-related diseases. Our lab has found a new lnc400, which is a ANP32A gene anti-sense RNA previously.ANP32A is a protein coding gene involved in numbers of cellular process, including proliferation, differentiation, apoptosis and tumor suppression etc. ANP32A acts as a component of histone acetyltransferases suppressor complex, involved in regulation of histone acetylation and phosphorylation process. Moreover, ANP32A protein expression level is promoted in Alzheimer's brain tissues, suggesting that ANP32A may be associated with pathogenesis and development.This study is to knock-down and over-express Inc400 in NPCs by taking lnc400 as study objection, NPC as material and lentiviral as mediates. Then detect lnc400 whether or not be influential to protein coding gene ANP32A in transcriptome level by qPCR and detect protein ANP32A expression level through western blot results show that Lnc400 has negative regulation to ANP32A on transcriptome level and translation level. When lnc400 is over-expressed or knocked-down in NPC, MAPK pathway, p53 signal pathway and Notch pathway have a series of changes with control group, influencing cellular process, cell component and molecular function.