Research On DNA Calculation Model Based On Molecular Beacon

In 1994, Adleman used DNA molecules to solve the seven nodes of a directed Hamilton path problem, which successfully opened the door of DNA calculation research field. DNA computer has become the new darling of the scientific research because of its characteristics of large storage space, high parallel and low power consumption.Since the emergence of DNA computing, it has a great progress and remarkable achievements, whether on the design of the model or in the realization of the hardware.The progress and the achievements provides a great help for further research and development of DNA computing.Molecular beacon is an oligonucleotide probe which is shaped similar to the "hairpin", with simple structure, high sensitivity and high specificity and other characteristics.Molecular beacon is originally used to measure the amount of target solution, after research and development by people, molecular beacon has become an important research tool for molecular biology, mathematics and other research areas. Yin zhixiang was the first to put forward to use the special structure of molecular beacon to solve combinatorial optimization problems, according to the solving method of Yin zhixiang, using molecular beacon as a carrier of the calculation, which made the following research, and tested its effectiveness by solve some practical problems.Firstly, according to the structural characteristics of molecular beacons,we combine molecular beacon with sticker model and use molecular beacon as the sticker chain in the sticker model, which generates the molecular beacons sticker model. Compared with the conventional molecular beacons model, the advantage of the model is does not need the participation of the biological enzyme and extension of the DNA chain; compared with the conventional sticker model, the advantage of the model is does not need to add a fluorescent probe to detect the result of DNA chains in actual operation. The molecular beacon sticker model is applied to solve the satisfiability problem, and gives specific examples of verification.Secondly, according to the structural characteristics of molecular beacon and the advantages of microfluidic chip technology, we combine the structural characteristics of molecular beacons with the advantages of microfluidic chip technology,.To achieve the calculation of molecular beacon on the microfluidic chip,we establish a new model of molecular beacon. The model makes up the shortcomings of molecular beacon in solution and solid surface,which is not easy to operate and has big error and so on. Iin-depth study of the molecular beacon provides more effective help. The microfluidic chips molecular beacon model is applied to solve 0-1 integer programming problem, and gives specific examples of verification.Finally, molecular beacon, sticker model and microfluidic chip technology are linked to construct molecular beacons sticker model based on microfluidic chip. The model makes up the shortcomings of the traditional model, such as complex operation, slow response and large error, and can be applied to more complex practical problems.