The QCM Immunosensor Network Based On ZigBee Protocol Apply To Tumor Marker Detection

Over the past 20 years, the cancer patients in our country have a tendency to be younger, and the morbidity and mortality of cancer are rising. Therefore, to improve the early detection rate of cancer becomes the most urgent needs for people to conquer cancer, and the most effective way to find cancer early is to detect cancer markers. At present, there are many limitations in the clinical detection of cancer markers, such as relying on large instruments, high cost, lacking of sensitivity and so on. This paper proposes a new method that is using the new type sensor technology called quartz crystal microbalances (QCM) to make the cancer biomarker immobilized nano ZnO modified QCM immunesensor combining nano materials and biological immobilized technology, and it was applied to the detection of cancer marker. Moreover, the QCM immunesensor can combine with wireless sensor network to construct the wireless QCM immunesensor network to realize monitoring the cancer markers the real-timely and remotely.In this dissertation, we use star network topology to construct the wireless QCM immunesensor network based on the ZigBee protocol. In this ZigBee network, the coordinator node and all terminal nodes are used CC2530 chip. Terminal nodes are connected with QCM immunesensor to measure the output frequency of QCM, and the frequency is transmitted to coordinator node via ZigBee network. The coordinator node sends the received data to PC via serial port for analysis and processing. In this paper, the wireless QCM immunesensor network is applied to the experiments of C-reactive protein (CRP) detection and breast cancer biomarker CA15-3 detection.In CRP detection experiment, we make the anti-CRP immobilized ZnO tetrapods QCM sensor. And the manufacturing process of this sensor includes the preparation of ZnO tetrapods (thermal evaporation method), the modification of QCM electrode using ZnO tetrapods (drop coating technique) and the immobilization of anti-CRP (improved physical adsorption method). In this experiment, the sensor is applied to the sensor network we have constructed. In CA15-3 detection experiment, we make the anti-CA15-3 immobilized ZnO nanorods QCM sensor. Aiming at the deficiencies (high temperature operation, high cost, nano film is not strong etc.) in CRP detection experiment, we improve the manufacturing process of QCM immunesensor. The improved manufacturing process includes ZnO nanorods film directly grow on QCM electrode (wet chemical method) and immobilization of anti-CA15-3. The wireless sensor network is also applied in CA15-3 detection experiment to realize monitoring CA15-3 real-timely and remotely. All obtained data in both two experiments show the sensors we make have advantages of good stability, linearity, reproducibility, specificity and fast response time.This work is supported by the National Natural Science Foundation of China under Grant No.61101060. I hope this research is able to speed up the application of QCM sensor in clinical detection of cancer bio marker. And based on this research, we will make the remote medical monitoring system to monitor cancer patients real-timely and remotely.