Image Fusion Technology Research Of Oocyte Dominant And Hidden Structure For Nuclear Transfer

Nuclear transplantation, featuring high gene infection rate, stable offspring, is a main method to realize somatic cell cloning. But it is not without its shortcomings: low embryo development rate, high death rate of the early embryonic. It is a time-consuming effort to obtain a case of living descendants from nuclear transplantation, and a large number of resources and money have to be invested. The growth of oocytes, the degree of trauma in the process of nuclear transplantation and the removing degree of nuclear are the three key factors that will influence the nuclear transplantation operation. A variety of invasive assistive technology of nuclear transfer do severe damage to oocytes, and oocyte screening depends largely on experience, lacking of quantitative detection methods, so oocyte activity can’t be guaranteed. Polarized light microscopy opens a new era of noninvasive way of nucleus research, but the information from polarized light system cannot provide the complete information needed for the oocyte development situation research.The clear image of internal structure in oocytes can been acquired under the polarized light microscope, and dominant structure have great imaging under biological inverted microscope. According to the properties of birefringence hidden structure of oocyte, oocytes ”hidden" structure information can be acquired by Abrio polarized light microscope, oocyte "dominant" structural information can be collected by Nikon Ti-E biological inverted microscope. Guided by the image fusion evaluation system, oocyte dominant and hidden structure information fusion can realized on the basis of image accurate registration. Extracting the spindle location information to realized the preliminary positioning of nucleus, extracting ZPT outline based on the combination of dominant and hidden structure feature, Multi-point measure zona pellucida to achieve oocytes ZPT values. The proposed image fusion algorithm of polarized light microscopic image and inversion microscopic image, facing high precision navigation nuclear transplantation, can clearly reveals the hidden structure of oocyte. But it only makes nuclear migration free from invasive assistive technology, nuclear transfer operation stillImage fusion technology research of oocyte dominant and hidden structure for nuclear transfer need to be handled by trained personnel, so this paper also focuses on the cell nucleus localization algorithm to realize great real-time nucleus positioning. Oocyte development situation is closely linked with thickness of zona pellucida, ZPT is the detective index of development status.This paper mainly introduces fusion algorithm of inverted microscopic images with polarized light images for high precision navigation of nuclear transplantation. It can be divided into the following several parts. First part introduces the research status of nuclear transfer micromanipulation assistive technology, and then research purpose and research content of this topic are determined. Second part introduction imaging principle of oocyte structure observation platform——inverted optical microscope and polarized light microscope, hidden structure feature and imaging principle. Third part analyzes the imaging characteristic of oocyte, realizes images registration from two systems based on pretreatment. Fourth part researches the image fusion methods suitable for mouse oocytes, which is guided by the image fusion evaluation system. Fifth part focuses on oocyte imaging platform: Nikon Ti-E biological inverted microscope and Abrio circular polarized light microscope, then information fusion algorithm of oocyte hidden and dominant structure and analysis of experimental results are introduced. Sixth part focuses on nucleus preliminary positioning algorithm and ZPT measurement algorithm, and then introduces the experiments of nucleus localization and ZPT measurement, experimental results analysis. The experimental results and related data show the proposed fusion algorithm of inverted microscope image with polarized light image is effective, the nuclear migration oocyte structure characteristics is clearly shown, the preliminary positioning of nucleus and ZPT measurement were realized, which can be used to detect oocyte development condition.