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Study On Properties Of Localized Surface Plasmon Resonance And Chitosan Micelles Fluorescence Applied To Chemical/Biological Sensor

Posted on:2014-11-20Degree:MasterType:Thesis
Country:ChinaCandidate:F LiuFull Text:PDF
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With the development of nano-science and nano-technology, more and more nano-materials have been researched. Gold nanorods as the basic of preparation composite structure has gradually become a kind of new functional material. Because of GNRs unique optical properties, has been used in the non-invasive diagnosis and treatment, biological sensing, detection and other fields, show the great application prospect. This article mainly involves the localized surface plasmon resonance (LSPR) properties of nanomaterials applied in the trace detection of actual samples. Different nanomaterials effect on the activity of cancer cells. Using the same gold nanorod detect three metals on the purpose of real-time continuous, and using chitosan colloidal detect the trace content of Fe3+by fluorescence and resonance light scattering. The Main research contents in thesis are as follows:(1) Using the method of seed growth synthesis GNRs with high yield rate, uniform size, length-diameter ratio is adjustable. Aspect ratio of GNRs is large depend on the Ag+and seed solution dosage. GNRs’s surface modification is enforced. Different aspect ratio GNRs solution mixed in certain proportion. Then selective detection target molecules-rabbit anti sheep/pig IgG. We apply the method to detection actual sample-patient serum. Randomly, selection10groups infected with rSjGST-32, TBAg serums have been made researched.(2) Using the seed growth synthesis method. Based on a single GNRs establish a method to detection three metal ions. Adding silver, copper, mercury ions to GNRs solution, can detect three metal ions including one, two or all the three metal ions. In order to eliminate the background interference, a layer of djustable Au thickness on the surface of GNRs. Adding AgNO3, Na2S2O3solution, form the nuclear-shell structure, lead to LPW red shift gradually, Then Cu(NO3)2, Na2S2o3solution continue to form Copper of GNRs solution. Simulaneously, LPW occurs blue shift gradually, Adding HgCl2, NaBH4solution, NaBH4reduced Hg2+to Hg (0), and Hg (0) and CuAgAu generate another structure,that lead to LPW red shift. The detection of mercury ion without copper ions, LPW occurs blue shift gradually, on the contrary, LPW gradually red shift in the presence of copper ions. So monitoring the LSPR change can achieve selectivity detection to three metal ions.(3) Under the heating conditions, Na2S2O3, Ag+interact with GNRs to form the AgS/Ag3S/AuAgS core-shell structure. Add the Cu2+and Na2S2O3solution generate Copper of GNRs. The surface of GNRs functionalized is as followed, stable-SH in alternative packages of GNRs surfactant bilayer. The surface of GNRs connection doxorubicin drug. GNRs-doxorubicin composite material are prepared. These materials’s absorption spectra are included in the700-900nm near infrared region. Biological tissues and cells is best light permeability to the wavelength of light absorption and scattering. The above materials were cultured breast cancer cells for24h, explore the different material effect the activity of cancer cells, and the state of getting into cells.(4) Through the heating time, concentration two aspects, optimization the experimental conditions can preparation chitosan gel with strong fluorescence. Using the fluorescence and resonance light scattering are analyzed the chitosan micelles, and found that colloid particle size are decrease gradually with increase of heating time, and fluorescence intensity occurs regularly weakening. Resonance light scattering intensity increasing. Using chitosan to trace detect Fe3+. By Fe2+and Fe3+reversible transform-ation. A comprehensive mechanism analysis of chitosan micelles interact with Fe3+were completed. Further chitosan material was applied to detection the Fe(Ⅲ) of actual sample-normal human serum, the average level of26.95μmol/L.
Keywords/Search Tags:Nanorods, Core-shell structure of gold nanorods, ChitosanGel, Na2S2O3
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