Fabrication And Characterization Of Gold Nanorods Bio-Sensing Micro Array

Biosensing microarrays attract great attention because of the characteristics of high throughput detection,small sample volume and simultaneous detection of various substances.Gold nanorods(GNRs)have anisotropic tunable optical properties and a unique surface plasmon resonance mode which can be used as sensing elements to construct biosensing microarrays with high selectivity,high sensitivity and low detection limits.However,there remains some challenges such as accurately controlling the position of GNRs,precisely controlling the arrangement between GNRs,and forming large-area array with uniform structures.Aiming at solving these problems,this work carried out researches on the fabrication and characteristic of GNRs bio-sensing microarrays systematically.The main research contents and research results are as follows:A new method for manufacturing grid-type GNRs self-assembly microarrays was proposed.Micro-nano processing technology was used to fabricate templates with micro-structures on the surface,and then template-assisted method and capillary self-assembly technique were combined to create large-area arrays.The effect of various factors on the self-assembled of GNRs was further explored by adjusting the aspect ratio,concentration and surface properties of GNRs and the humidity of the assembled environment,which provided an effective method for the fabrication of orderly arranged GNRs self-assembly microarrays.A new method for manufacturing circular GNRs self-assembly microarray was proposed.A micron-sized hydrophilic array was fabricated on the glass surface by the wettability differential interface treatment method,and then GNRs were selectively self-assembled on the hydrophilic array of the glass surface by solvent evaporation method.The robustness and universality of the method were verified by adjusting the size and shape of the hydrophilic array.GNRs self-assembly microarrays of various sizes and structures with a yield of up to 100% had been fabricated successfully.In addition,the sensing properties of GNRs microarrays were tested.Conductivity test results showed that the grid-type GNRs array had extremely high conductivity and excellent ohmic characteristics.The Raman test results showed that the GNRs array had a very high Raman enhancement factor,and the GNRs with different arrangement modes had different enhancement factor.The specifically detection of immunoglobulin IgG confirmed that the GNRs array could be used as a Raman-active substrate to detect biomolecules with high sensitivity.The cell culture phenomenon proved that the grating GNRs array could be used to induce cell-oriented growth.The GNRs microarrays proposed in this paper have the advantages of simple manufacturing process,high yield,precise regulation,good sensing performance and good biocompatibility,and provides a practical operation scheme for the manufacture of biosensing microarrays.