AMPK In Muscle-derived IL-6Inhibition Of Insulin Role Of Resistance Occurred

AMPK signalling pathways are studied in this paper the muscle source sex IL-6to inhibit the action of the insulin resistance occurs; Discuss blood, muscle, liver,and adipose tissue in movement, high fat diet and shRNA (short hairpin RNA)intervention, the study of AMPK organizations il-6expression quantity and muscleorigin of mutual influence and change; IL-6and movement and muscle origin;AMPK improving mechanisms of insulin resistance in rats; Reveal AMPK could bemuscle source sex IL-6important transduction factor inhibiting insulin resistance.Research purposes: under the intervention of aerobic endurance training of highsugar and high fat diet rats experiment, study muscle source of il-6in the role ofinsulin resistance, explore amp activated protein kinase (activated protein kinases,AMPK) as the protein pathways to inhibit the action of the insulin resistance.Experimental method and steps of:72male SD rats, about4weeks of age, bodyweight100and10g, randomly divided into nine groups by weight (A-I), eachgroup of eight only. Group A: quiet normal feed group; Group B: quiet high fat feedgroup; Group C: movement high fat feed group; Group D: movement high fat feedshRNA group; Group E: movement, high fat feed blank carrier group; Group F: quiethigh fat feed shRNA group; Group G: quiet high fat feed blank carrier group; Hgroups: quiet normal feed shRNA group; Group I: quiet normal feed blank carriergroup. Sports aerobic training group animals need to run, take a day off every week,sports training and6days, starting to16m/min running speed, time for50minutes,training for8weeks; Need injections of SD rats by tail vein injection of shRNAsimultaneously or blank carrier, shRNA or blank carrier mice tail intravenousinjection earlier in the day before the official start of the training, every movementthree times after2weeks after the day of rest injections of shRNA or blank carrier;Aerobic training every week,1m/min running speed, prolonged time5minutes;Until at the end of8weeks, movement speed is23meters/minute, time was85minutes. End of the experiment, all the SD rats by using anesthesia sampling.Respectively to extract rat blood, muscle, fat, abdominal fat) and partial livercryopreservation reanalysis data. Then compare different groups, fasting glucose, fasting insulin and glucose infusion rate changes, or blank carrier injection shRNAcontrast, the comparison group and blank group gene silencing organization serumIL-6levels, organizations IL-6mRNA level. Data analysis and detection of IL-6inhibition of insulin resistance and AMPK formation.Experimental test indicators:1: fasting glucose, fasting insulin and glucose infusion rate in each groupexpression.2: The serum concentration of IL-6, IL-6mRNA level organizations.3: IL-6in the muscle, liver and adipose tissues of the control amount4: AMPK in muscle, liver and adipose tissue contrast expressionConclusion:(1) high-fat diet can induce insulin resistance, insulin resistance in high-fat dietrats formed the liver and adipose tissue was significantly activated AMPK.(2) the formation of a quiet and high-fat diet insulin resistance state, the liverand adipose tissue gene expression of IL-6strongest; while exercise stress is greatlyimproved skeletal muscle gene expression of IL-6.(3) rats by aerobic training can improve and prevent insulin resistance,inhibition of insulin resistance movement occurs in a certain extent by IL-6andwork.(4) Games induced changes in AMPK protein, inhibition of insulin resistance inthe process of forming, with the AMPK.(5) shRNA interference effect is obvious, to achieve the desired experimentaldesign requirements during the experiment, IL-6can effectively control the AMPKprotein expression, but IL-6gene silencing is not necessarily activate AMPK.(6) AMPK in muscle-derived IL-6inhibition of insulin resistance in the processof gene transcription regulation of energy metabolism, in order to further confirmAMPK is mediated by IL-6inhibition of insulin resistance factor signal transductionsubsequent experiments provided evidence.