| Background and Objective：In the gene era, in the life science research withwhole system as the research object, by genomics, proteomics, metabolomics as arepresentative of systems biology has become the main trend. Metabolomics throughthe measurement of samples to reflect all the metabolites of different organisms of thestimulus reaction, use metabolic fingerprint for life system for research. Relative tothe level of gene research, metabolomics is sensitie higher, integrity, stronger, test andanalysis is simple, fast. In previous studies, we already preliminary evidence of theathletes metabolic fingerprint contains the athletes’ level of information, and toestablish a high level of man short swimming performance prediction metabolomicsmodel. The SD rats for research object, explorecan use youth rats (4weeks olds) urinemetabolic fingerprint to identify with swimming talent of the rats that is afterlong-term training, at14weeks olds can obtain good swimming performance, andthrough the comparison with biochemical method to analysis based on the analysis ofthe nuclear magnetic resonance (NMR) metabolomics applied selecting athletictalents the feasibility and the superiority.Method：Twenty four male SD rats were taken as the research objects. Theirurine was collected at4weeks old and analyzed by NMR, blood samples for testinghemoglobin (Hb) and serum testosterone (ST). After10weeks gradually increasingload swimming training, the24rats were tested swimming ability. According toexhaustive swimming time, they were divided into the1st-3rd group and4th-24thgroup,1st-12th group and13th-24th group, the test is completed, have two days off,put to death the ends, take right hind leg calf muscle test succinic acid dehydrogenase(SDH) and lactate dehydrogenase (LDH) content. Partial least square analysis wasused to do pattern recognitions and variable importance calculation on the1H NMRdata from these rats’ urine samples.Results:1st-3rd group and4th-24th group rats of the Hb, ST, SDH, LDHrespectively is:152.3±9.5:165.73±74.42g/L、2.47±0.06:2.48±0.34nmol/L、29.77±7.48:35.38±38.23U/mgprot、0.58±0.4:0.62±0.29U/mgprot, no significant difference;1st-12th group and13th-24th group rats of the Hb, ST, SDH, LDH respectively is:153.72±21.72:174.39±65.76g/L、2.47±0.33:2.49±0.13nmol/L、33.46±40.05:35.9±37.72U/mgprot、0.59±0.39:0.64±0.27U/mgprot，the results also no significant difference; Metabolomics pattern recognition results show that the1st-3rd group and4th-24th group,1st-12th group and13th-24th group’s sample of metabolic fingerprinthave obvious difference, the sample is clearly separated, and the same group thesample is appeared together.Conclusion: Young rats urine metabolic fingerprint can reflect the rat swimmingpotential difference, relative to the use of the individual or a few index selectingathletic talents biochemical method, metabolomics has its unique advantage. |
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