| Zanthoxylum armature DC. is a plant of Zanthoxylum from the family of Rutaceae, and in China, the plant has been used for a very long time. Its root, stem, leaves, fruits and seeds all can be considered as medicinal parts. In recent years, scholars from all the world have isolated lots of chemical compositions from Zanthoxylum armature DC., including alkaloids,lignans, amides, flavonoids and so on. Studies on its pharmacology had proved this plant could show analgesic effect, anti-inflammatory effect, hepatoprotective effect, antibacterial effect, antioxidant. Until now, there are many studies on chemical constituents from petroleum ether, hexane, Et OAc extraction of Zanthoxylum armature DC., while few studies on chemical constituents from water extraction. Some researches had proved Et OAc extraction of the plant is effective in resisting inflammation and easing the pain, while alkaloids of Et OAc extraction is more effective. Based on this theory, this experiment has studied on Chemical Constituents in Et OAc and water extracts of Zanthoxylum armature DC..It aimed to isolate monomeric compound from this plant and provided material to further study on Pharmacological activity.In this paper, Et OAc and water extractions of Zanthoxylum armature DC. were isolated by silica gel colum chromatograph, Sephadex LH-20, MCI, HW-40, C18 and TLC. The structures of these compounds are elucidated on the basis of 1H-NMR, 13C-NMR, ESI-MS and other spectral techniques. Fifty seven compounds were obtained from these two extractions, fifty one were identified by spectral techniques. They are 4-hydroxy-2, 6-dimetho-xyphenyl 1-O-α-L-rhamnopyranosyl(1→6)-β-D-glucopyranoside(1), 2-methoxy-4-hydroxy-lphenyl-1-O-a-L-rhamnopyranosyl-(1’’→6’)-β-D-glucopyranoside(2),(-)-(7R,8S)-guaiacylg-lycerol-8-O-β-D-glucopyranoside(3), Isotachioside(4), threo 1-(4-hydroxy-3-methoxy)-phe-nyl-1,2,3-propanetri(5), 7-(3-hydroxy-5-methoxyphenyl)propane-7,8,9-triol(6), 1-(4-hydrox-yphenyl)propane-1,2,3-triol(7), candicine(8), threo-3-methoxy-5-hydroxy-phenylpropanetri-ol-8-O-β-D-glucopyranoside(9),tyrosine(10), thero-1-C-syringylglycerol(11),(2-methoxy)-hydroquinone 4-β-D-glucopyranoside(12), 2,6-dimethoxy-4-hydroxyphenyl-1-O-glucopyran-oside(13), cuneataside D(14), erythro-1-C-syringylglycerol(15), canthoside C(16), meliad-anloside A(17), leonuriside A(18), xylocoside A(19), aenosine(20), 3,5-dimethoxycinnam-yl alcohol 4-O-β-D-glucopyranoside(21), 3-hydroxy-2-{4-[(1E)-3- hydroxyprop-1-en-1-yl]-2-methoxyphenoxy}propyl-β-D-glucopyranoside(22),(1’S,6’R)-8’-hydroxyabscisic acid β-D-glucoside(23), 4’-dihydrophaseate sodium(24), coniferin(25), nikoenoside(26), phydroxy-phenethyl-β-D-Glucoside(27), guanosine(28), chlorogenic acid(29), psoralenoside(30),isopsoralenoside(31), vanillic acid(32),(7S,8R)-guaiacylglycerol-α-ferulic acid ether-7-O-β-D-glucopyranoside(33),isodecarine(34),(+)-pinoresinol-di-3,3-dimethylallyl ether(35), pla-nispine A(36), 1,2-benzenedicarboxylic acid diisooctylester(37), phillygenin(38),syringaresinol(39), de-4’-O-methylyyangambin(40), 6-acetonyl dihydrochel-erythrine(41),dictamnine(42),(-)-fargesin(43),(+)-fargesin(44), eudesmin(45), allocrypto-pine(46),8-acetonyl-dihydrofagaridine(47), escholidine perchlorate(48), kobusin(49), 1,2,3-trihydroxy-1-(3,4-methyenedioxyphenyl) propane(50), norchelerythrine(51).Among them, compounds 2, 9, 33 are new; compounds 1, 3~7, 11, 14~17, 19, 22~24, 26,27, 30, 31, 38, 48 and 50 were isolated from family Rutaceae for the first time. Compounds12, 20 and 28 were isolated from genus Zanthoxylum Linn for the first time. Compound 8, 10,13, 21, 25, 29, 35, 37, 41, 46 and 47 were firstly reported from Zanthoxylum armature DC.Structural identification of remaining monomers compounds and the antibacterial activities of all compounds will be done later. |
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