The Research Of Chemical Constituents Isolated From The Ethyl Acetate Extracts Of Zanthoxylum Nitidum(Roxb.) DC. Leaves

Objective:To study the bioactive chemical constituents of ethyl acetate extracts of Zanthoxylum nitidum（Roxb.）DC.leaves.Methods:Zanthoxylum nitidum leaves were dried naturally and crushed to powder,then the sample powders were reflux extracted four times with 70%ethanol.Each reflux were dissolved in pure water and extracted with petroleum ether,ethyl acetate and n-butanol to obtain different polar extracts.The ethyl acetate extracts were separated and purified by silica gel column chromatography,polyamide column chromatography,RP-18 column chromatography,Sephadex LH-20 column chromatography,PTLC,RPLC recrystallization and other methods.The compounds structures were elucidated by spectroscopic techniques and chemical methods,such as ¹H NMR,¹³C NMR,ESI-MS,IR,UV.Results:Twenty compounds were isolated from the ethyl acetate extracts of Zanthoxylum nitidum（Roxb.）DC.Leaves,such as flavonoids,alkaloids,lignans,and so on.Eighteen compounds were identified,includingβ-sitosterol（1,LMYL-1）,hesperetin（2,LMYL-2）,ethyl caffeate（3,LMYL-4）,3,4-dihydroxy benzoic acid（4,LMYL-5）,4-methoxyquinolin-2-one（5,LMYL-7）,pinoresinol（6,LMYL-9）,medioresinol（7,LMYL-10）,syringaresinol（8,LMYL-11）,N-benzoyl-L-Phenylalaninol（9,LMYL-12）,N-trans-coumaroyl tyramine（10,LMYL-13）,L-sesamin（11,LMYL-14）,hexadecanoic acid（12,LMYL-15）,indolyl-3-carboxy-lic acid（13,LMYL-19）,diosmetin（14,LMYL-20）,zhebeiresinol（15,LMYL-25）,vitexin（16,LMYL-29）,isoscopletin（17,LMYL-33）,loliolide（18,LMYL-6）.Compounds 3,4,5,6,7,8,9,10,11,12,13,15,16,17and 18 were isolated from Zanthoxylum nitidum for the first time.Conclusions:The study provids a scientific and theoretical basis for the development and application of Zanthoxylum nitidum（Roxb.）DC.leaves.