Effect Of Parthenolide On Intimal Hyperplasia After Balloon Injury And The Underlying Mechanism

Objective To detect the effect and underlying mechanism of Parthenolide(PN) on intimal hyperplasia in iliacartery of rabbit after balloon injury.Methods The inflammatory model of intimal hyperplasia was established by a combination of balloon injury and high-fat diet. After 1 week of high-fat feeding, 30 male white rabbits(2.0-2.3) kg were randomly divided into 6 groups: A:Sham+NS: rabbits got 0.9% normal saline(NS) after sham operation(n=5); B:Sham+DMSO: rabbits got 4%DMSO after sham operation; C:balloon injury+NS: rabbits got 0.9%NS after balloon injury(n=5); D:balloon injury+DMSO: rabbits got 4%DMSO after balloon injury(n=5); E:balloon injury+PN low: rabbits got PN at 1mg/kg after balloon injury(n=5); F:balloon injury+PN high:rabbits got PN at 2 mg/kg after balloon injury(n=5). Drugs were intraperitoneal injected once per day until sacrifice. After high-fat diet for four weeks, 30 rabbits were all seleceted for this study. The intima-media thickness, the expression of NF-κB, IL-6, Caspase-1, IL-1β, and the levels of IL-8, TNF-α, TC, TG, LDL, HDL in serum were measured.Results The results of Comparison between group A vs group B and group C vs group D showed that there was no statistical significance(P>0.05). Compared with group B(Control gourp), the thickness of intima, the expression of NF-κB, IL-6, Caspase-1, IL-1β, IL-8 and TNF-α in group D(Model gourp) were significantly increased(P<0.05). The amount of NF-κB, IL-6, Caspase-1, IL-1β, IL-8 and TNF-α was significantly decreased in F group(High-dose group) compared with D group(P<0.05). The level of lipid was not statistically significant in different groupsConclusion Neointimal hyperplasia is suppressed by PN after balloon injury, the potential mechanism maybe associate with its anti-inflammatory role in inhibiting the activation of NF-κB and Caspase-1 and down-regulating the expression of inflammatory factors.