Relationship Of Toll-like Receptor 1 Polymorphism With Gastric Precancerous Lesions

Background: The morbidity and mortality of gastric cancer rank the second in the world, In our country, the new cases of gastric cancer increase even nearly one million each year. A large number of studies indicate that intestinal metaplasia and intraepithelial neoplasia are precancerous of gastric cancer.Toll like receptor 1(TLR1) polymorphism is related with many diseases,however,only few studies have been performed on its relationship with gastric precancerous lesions.Aims:To investigate the relationship between TLR1 rs4833095 polymorphism and gastric precancerous lesions.Methods: A total of 432 patients with gastric precancerous lesions(including 84 intraepithelial neoplasia,348 intestinal metaplasia) from Dec.2008 to Dec.2014 at Qingdao Municipal Hospital were enrolled,and 540 healthy subjects were served as controls.TLR1 rs4833095 polymorphism was determined by DNA sequencing,and Helicobacter Pylori(H.Pylori) infection was deteted. Relationship of TLR1 gene polymorphism with gastric precancerous lesions was analyzed by multiple Logistic regression models after adjusting for age,sex,H.Pylori infection and smoking status.Results: Significant differences in the frequencies of GG、GA and AA genotype on TLR1 rs4833095 were found between disease group and control group(χ2=19.966,P=0.000).The frequency of AA genotype in disease group was significant higher than that in control group(17.6% vs 8.9%,χ2=16.336,P=0.000). Compared with GG genotype,patients with GA genotype were obviously more susceptible to gastric precancerous lesions(OR=1.329,95%CI:1.002—1.762).Compared with GG+GA genotypes with negative H.Pylori, patients with TLR1 rs4833095 AA genotype and positive H.Pylori infection had much higher susceptibility to gastric precancer lesions(OR=3.617,95%CI:2.147-6.092).Conclusion: TLR1 rs4833095 polymorphism is positively associated with susceptibility to gastric precancerous lesions.