Preliminary Study On Polymorphism Of Toll-like Receptor 9 Gene In Patients With Condyloma Acuminatum

Condyloma Acuminate is a kind of Sexually Transmitted Disease (STD). In recent years, the incidence of catching this disease appears to have an upward trend, and pathogenesis of Condyloma Acuminatum is not yet thoroughly clear. There is, so far, no effective anti-viral treatment for Condyloma Acuminate, and no satisfactory solution to the recurrence. Different individuals have different responses to HPV infection, suggesting that immunity, particularly, the host cell immunity, has a certain impact in the occurrence of Condyloma Acuminate. It has been widely recognized that the change of cellular immune function closely relates to the occurrence of Condyloma Acuminate. That the body can not build an effective immune response is the main reason for HPV persistent infection; so to explore the immune mechanisms of Condyloma Acuminate has important oretical and clinical significance.Toll-like receptor (TLR) is the first recognition receptor of the inherent immunity, which is a defending response caused by direct perception of microbial pathogen. This kind of receptor plays an important part in inherent immunity defense, and can also adjust adaptive immunity, which makes it a bridge of connecting inherent immunity and adaptive immunity. TLR9 mainly identifies bacteria and virus genome DNA and synthetic CpG ODNs, and it has an important role in the pathogenesis of anti-infection, anti-tumor, mediated cell apoptosis and autoimmune disease.ObjectiveThis study aims at investigating whether polymorphism in the TLR9 gene differentially affect the incidence and the clinical course of Condyloma Acuminatum.MethodsFollowing informed consent from all patients and healthy volunteers,2ml of peripheral blood was collected from each of 63 Guangdong Condyloma Acuminatum patients and 23 unrelated healthy Guangdong individuals admitted to The Fourth Affiliated Hospital of Jinan University. The patients were divided into groups in terms of the primary and recurrent Condyloma Acuminatum patients. With DNA samples from all subjects prepared, all single nucleotide polymorphisms of TLR9 was tested through polymerase chain reaction and direct sequencing. ResultsThere are 4 SNPs in TLR9:SNP1,SNP2,SNP3 and SNP4. SNP1 was located in intron 1 region, SNP2,SNP3 and SNP4 located in exon2. The registration number of SNP1 is rs352139 in NCBI database, and that of SNP2 is rs352140. SNP2 is non-synonymous cSNP. Based on a SNP map that had been constructed, we also constructed a haplotype map of TLR9. The frequency of alleles and genotypes in chinses was revealed as well. But there were no significantly increase in patients compared with controls on SNP1 and SNP2. There are statistical differences between the two groups of the primary and recurrent Condyloma Acuminatum patients on Th/Ts. Two sites of three genotypes were not statistically significant compared with Th/Ts. SNP3 and SNP4 were observed only in the patients, therefore, we don't have analyzed these two sites.ConclusionsTLR9 genes exist as four single nucleotide polymorphisms (SNPs), respectively, SNP2, SNP1, SNP3, SNP4, in Guangdong Han ethnic population. Compared with the control group, no significant increase of single nucleotide polymorphisms in TLR9 can be seen in the patient group. But the data of TLR9 polymorphism in Guangdong Han Ethnic Group will significantly contribute to the improvement of research on disease association and basic immunology.