The Expression Of Integrin α2β1 And CD44V4 In Carcinoma And The Relationship Of Them

Nowadays, gastric carcinoma that belong to digestive tract carcinoma is one of most develop cancer. It is a very complicated process and involve many complicated mode of action. Many molecule involve in and integrin α2β1 and CD44V4 may also participation in it.The experimental test which were expressed by integrin α2β1 and CD44V4 in gastric carcinoma tissue and normal gastric tissue. Research the realationship between integrin α2β1 and CD44V4 and clinico-pathological characteristics and the realationship of themselves. Discuss the role of integrin α2β1 and CD44V4 in the progress of gastric carcinoma. Assessing the prognosis of gastric carcinoma patience. That can provide theortical basis for treatment of gastric cancer.ObjectiveThe experimental test the expression of integrin α2β1 protein and m RNA and CD44V4 protein and m RNA in gastric carcinoma tissue and normal gastric tissue respectively. Using statistical methods Analyze the experimental data. Research the realationship between integrin α2β1 and CD44V4 and clinico-pathological characteristics and the realationship of integrin α2β1 and CD44V4.MethodsCollection 85 cases of gastric carcinoma tissue which was diagnosised by pathological and 50 cases of normal gastric tissue which was away 10 cm from gastric carcinoma tissue in November 2012 to March 2014 to collect a number of hospitals in Chengde. Testing the expression of integrin α2β1 protein and m RNA and CD44V4 protein and m RNA in gastric carcinoma tissue and normal gastric tissue by the immumohistochemical method and RT-PCR. Analysis the date of the experimental by statistical methods. Combined with clinical pathological characteristics. Then drawing a conclusion by using above all of them.Results1 Integrin α2β1 protein and CD44V4 protein expression in normal gastric tissue was obviously lower than in gastric carcinoma tissue. The difference that came from the experiment of date was statistically significant(P<0.05, P<0.05).1.1 Integrin α2β1 protein expressed in well-moderately differentiated gastric carcinoma was obviously lower than in poorly differentiated gastric carcinoma(P<0.05). Integrin α2β1 protein expressed in TNM stage I-II was obviously lower than in TNM stage III-IV(P<0.05). Integrin α2β1 protein expressed in the group of infiltrating serous layer was obviously lower than in the group of unserous layer(P<0.05). Integrin α2β1 protein expressed in the group of lymph node metastasis was obviously lower than in the group of lymph node non-metastasis(P <0.05).1.2 CD44V4 protein expressed in well-moderately differentiated gastric carcinoma was obviously lower than in poorly differentiated gastric carcinoma(P<0.05). CD44V4 protein expressed in TNM stage I-II was obviously lower than in TNM stage III-IV(P<0.05). CD44V4 protein expressed in the group of infiltrating serous layer was obviously lower than in the group of unserous layer(P<0.05). CD44V4 protein expressed in the group of lymph node metastasis was obviously lower than in the group of lymph node non-metastasis(P<0.05).There was no significant differences that integrin α2β1 protein and CD44V4 protein expressed in gastric carcinoma tissue from the group of different gender and the group of ages(P>0.05, P>0.05).2 Integrin α2β1 m RNA and CD44V4 m RNA expressed in normal gastric tissue was obviously lower than in gastric carcinoma tissue. The difference that came from the experiment of date was statistically significant(P<0.05, P<0.05).2.1 Integrin α2β1 m RNA expressed in well-moderately differentiated gastric carcinoma was obviously lower than in poorly differentiated gastric carcinoma(P<0.05). Integrin α2β1 m RNA expressed in TNM stage I-II was obviously lower than in TNM stage III-IV(P<0.05). Integrin α2β1 m RNA expressed in the group of infiltrating serous layer was obviously lower than in the group of unserous layer(P<0.05). Integrin α2β1 m RNA expressed in the group of lymph node metastasis was obviously lower than in the group of lymph node non-metastasis(P <0.05).2.2 CD44V4 m RNA expressed in well-moderately differentiated gastric carcinoma was obviously lower than in poorly differentiated gastric carcinoma(P<0.05). CD44V4 m RNA expressed in TNM stage I-II was obviously lower than in TNM stage III-IV(P<0.05). CD44V4 m RNA expressed in the group of infiltrating serous layer was obviously lower than in the group of unserous layer(P<0.05). CD44V4 m RNA expressed in the group of lymph node metastasis was obviously lower than in the group of lymph node non-metastasis(P<0.05).There was no significant differences that integrin α2β1 m RNA and CD44V4 m RNA expressed in gastric carcinoma tissue from the group of different gender and the group of ages(P>0.05, P>0.05).3 The correlation of integrin α2β1 and CD44V4 expressed in gastric carcinoma tissue3.1 The correlation of the expression of integrin α2β1 protein and CD44V4 protein is r=0.517(P<0.05).3.2 The correlation of the expression of integrin α2β1 m RNA and CD44V4 protein is r=0.752(P<0.05).Conclusion1 That integrin α2β1 protein and CD44V4 protein expressed in gastric carcinoma tissue have significant differences. And both of them have close contact with clinical pathological characteristics such as infiltrating depth, TNM stage, lymph node metastasis, differentiation degree.2 The relative expression of integrin α2β1 m RNA and CD44V4 m RNA have significant differences in gastric carcinoma tissue. And both of them have close contact with clinical pathological characteristics such as infiltrating depth, TNM stage, lymph node metastasis, differentiation degree.3 There was a positive correlation between integrin α2β1 and CD44V4.