The Effect Of Histone On The Intestinal Mucosal Barrier Function Of Mice

Objective::1.To research the effect of Histone on the intestinal mucosal barrier function of mice (including intestinal mucosal permeability and bacterial translocation);2,.To evaluate the effect of anti-histone antibody on the protection of intestinal mucosa barrier in order to research the toxic effects of histones and provide clues to response.Methods 50 female C57BL/6 rats randomly divided into 5 groups, Choose 6 to 7 weeks health male C57BL/6 mice, weight in 20 to 25 g, control group (n=10), experiment group 1(n=10), experiment group 2 groups (n=10), treatment group1 (n =10), treatment group 2 (n=10). Each treatment:the control group (0 mg/kg), experiment group 1 (30 mg/kg histone), experiment group 2 (60 mg/kg histone); Treatment group 1 (histone 60 mg/kg+ anti-histone antibody 10 mg/kg). Treatment group 2:(histone 60 mg/kg+ anti-histone antibody 20 mg/kg). Kill the mice and get samples after an hour, By detecting the Fluorescence intensity of FITC-Dextran(FD4) in the portal vein to evaluate the permeability of intestinal mucosa. Take mesenteric lymphatic tissue, liver and lung tissue to detect the intestinal bacterial translocation rate under the condition of bacteria. Parallel intestinal mucosal tissue with the pathology examination.Result Compared with control group, experimental group intestinal mucosal permeability, bacterial translocation rate is increased, and as the amount of histone concentration increases, the permeability of intestinal mucosa and bacterial translocation rate has increased trend, while the treatment group permeability of intestinal mucosa, bacterial translocation rate compared with the experimental group decreased obviously, and the small intestine tissue pathology observation, control group almost no damage, the experimental group are obvious necrosis, intestinal tissues and increased with the concentration of histone, the greater intestinal tissue injury, treatment group has necrosis, intestinal necrosis but the experimental group was significantly reduced, no significant bleeding.Conclusion Histone can destroy the function of intestinal mucosa in mice, increase the permeability of intestinal mucosa in mice, and the concentration, the greater the permeability of intestinal mucosa, bacterial translocation rate is higher, the tissue injury is more serious, and histone antibodies have protective effect to the intestinal mucosa, helps to reduce the damage of intestinal mucosa.