Expression And Clinical Significance Of Serum Chemokines In Patients With Non-small Cell Lung Cancer

Objective:To detect the serum expression of 6 chemokines ITAC, Fractalkine, MIP-3α, IL-8,MIP-1α, MIP-1β in patients with NSCLC and health controls group. And analyze their association with the pathological type and metastasis of NSCLC as well as the correlations among these chemokines. To provide a new theoretical basis for the explore of the impact of chemokines in the progression and metastasis of NSCLC.Methods:Thirty of healthy controls and thirty-five newly diagnosed and untreated patients with NSCLC were enrolled. The NSCLC group included 13 cases had metastasis and 22 cases with non-metastasis, 18 cases of squamous cell carcinoma and 17 cases of adenocarcinoma. The expression of 6 kinds of serum chemokines were detected by Luminex technology. The results were analyzed by SPSS 17.0 software.Results:(1). The serum levels [M(QR)] of IL-8, Fractalkine, MIP-3α in control group were2.01(0.95), 61.46(74.81), 8.08(5.87) and in patients with NSCLC were 5.10(4.15),119.60(119.94), 10.47(9.01) respectively. There were statistically significant between the two groups(Z=﹣4.665, P<0.001; Z=﹣3.930, P<0.001; Z=﹣3.034, P=0.002).(2).The expression of 6 serum chemokines were no statistical difference between the two groups(P>0.05). But except for IL-8, MIP-1α, the expression tendency of Fractalkine, MIP-3α, MIP-1β, ITAC in patients with metastasis were slightly higher than that of without metastasis group(but P>0.05).(3). In lung adenocarcinoma the expression level of serum MIP-1β was significantly higher than that of lung squamous cell cancer patients[18.32(12.27) vs. 13.72(7.31), Z =﹣2.212, P =0.027).(4). The expressions of MIP-3α and Fractalkine were positively correlated in two groups(r=0.612, P<0.001; r=0.766, P<0.001). The expressions of IL-8 and MIP-1α were positively correlated in NSCLC group(r=0.411, P=0.041). And in healthy control group the expressions of IL-8 and MIP-3α were positively correlated(r=0.456, P =0.011).Conclusions:(1). The expressions of serum IL-8, Fractalkine, MIP-3α in patients with NSCLC compared with control group were significantly higher. They maybe promoted the development and occurrence of NSCLC. It may be used for auxiliary diagnosis and improve the positive diagnosis of NSCLC by combined detection of them with conventional tumor markers.(2). In lung adenocarcinoma the expression level of serum MIP-1β was significantly higher than that of lung squamous cell cancer patients. It suggested that the expressions of chemokines might varies with the different pathological type of NSCLC.(3). The expression tendency of ITAC, Fractalkine, MIP-3α, MIP-1β in patients with metastasis were slightly higher than that of non-metastasis group(but P>0.05). It suggested these chemokines may be associated with the metastasis of NSCLC. Blocking or down-regulating the expression of these chemokines might prevent the metastasis of NSCLC.(4). The levels of serum MIP-3α and Fractalkine were positively correlated in both two groups. It suggested there maybe overlapping cooperation among each chemokine.The combined detection of multiple chemokines contribute to make an objective and overall evaluation of disease.